DETECTION OF MYCOBACTERIUM TUBERCULOSIS DNA IN SPUTUM SAMPLES WITH MPB 64 GENE PRIMERS USING POLYMERASE CHAIN REACTION.
Journal Title: International Journal of Advanced Research (IJAR) - Year 2018, Vol 6, Issue 4
Abstract
Tuberculosis (TB) is preventable and curable, but it can lead to death if no actions are taken. In order to prevent transmission, it is necessary to identify infectious TB patients in a timely manner. The present study was aimed to detect the mycobacterium tuberculosis DNA in sputum samples with MPB 64 gene primers using PCR. Hundred patients were enrolled in the study based on inclusion and exclusion criteria. Sputum samples were collected and processed for the required procedure. Ziehl-Neelsen staining was done and slides were examined under microscope. DNA was extracted from the samples and amplified using PCR. Staining results of both spot and morning samples was compared under microscope with grading.
Authors and Affiliations
Ajab Singh Choudhary, Dakshina Bisht Jyoti Sharma, Jyoti Choudhary.
Keywords
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- EP ID EP290487
- DOI 10.21474/IJAR01/6955
- Views 69
- Downloads 0
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