DETECTION OF RESISTANT PHENOTYPES OF PSEUDOMONAS AERUGINOSA IN TERTIARY CARE HOSPITAL
Journal Title: International Journal of Microbiology Research - Year 2016, Vol 8, Issue 11
Abstract
Background: Pseudomonas aeruginosa cause infections of all sites and is common cause of nosocomial infections. Objectives: Detection of resistant phenotypes of P.aeruginosa clinical isolates in rural tertiary care hospital. Materials and methods: A total of 88 P.aeruginosa were included in the study and antibiotic susceptibility of the isolates was determined by Clinical laboratory and standard institute [CLSI] guidelines. Betalactames, aminoglycosides and quinolones resistant phenotypes of P.aeruginosa are detected on the basis of antibiotic susceptibility profile. Extended spectrum betalactamases (ESBL), AmpC betalactamases and Carbapenemases are detected using standard microbiology guidelines. Result: Out of 88 P.aeruginosa isolates, majority were isolated from sputum followed by urine and endotracheal tube. Betalactams resistant phenotypes: Out of 88, 71 (81%) were natural wild strain, 4(5%) were ESBL, 3 (4%) were carbapenamase, 1 (1%) was penicilinase, oprJ, oprN, and D2 resistant phenotype. Aminoglycosides resistant phenotype: Out of 88, 64 (73%) were natural wild strain, 6 (7%) were G phenotype, 5 (6%) were impermeability phenotype, and 1 (1%) was GNtT phenotype. Quinolones resistant phenotype: Out of 88, 74 (84%) were natural wild strain, 4(5%) were IV phenotype, and 2 (2%) were efflux phenotype. Conclusion: Early identification of resistant phenotypes of P.aeruginosa based on antimicrobial susceptibility result would guide clinician to start early appropriate therapy that lead to good clinical outcome, and to reduce spread of nosocomial infections
Authors and Affiliations
SWETA S. OZA, SANJAY J. MEHTA, SUNIL G. OZA
Keywords
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