DETECTION OF SENSITIVE FOOD PATHOGENS IN BANANA, COLD MEAT AND MILK BY PCR AMPLIFICATION BASED TECHNIQUE
Journal Title: Journal of Biological and Scientific Opinion - Year 2014, Vol 2, Issue 5
Abstract
This study was carried out to detect Escherichia coli, Klebsilla spp. and Streptococcus spp. isolated from food samples (banana, cold meat and milk). The homogenized food samples were subjected to biochemical and molecular techniques. Specific primers for virulent genes of three different bacterial strains were designed and subjected to polymerase chain reaction. PCR reaction was performed with human primers also in order to confirm the nonspecific amplification. Varying concentrations of DNA (100 ng, 50 ng, 20 ng, 5 ng and 1 ng) were chosen from each bacterial strain and PCR was carried out. It was observed that E. coli and Streptococcus were active in all the concentrations including 1 ng. However, Klebsiella did not produce amplification at 1 ng. The results obtained suggest that E. coli and Streptococcus could be detected and remain active even in 1 ng of the DNA sample.
Authors and Affiliations
Brindha Durairaj, Kirupa Devaki , Santhoshkumar Muthu
Keywords
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- EP ID EP99954
- DOI 10.7897/2321-6328.02566
- Views 146
- Downloads 0
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