Determination of content of travoprost in travoprost eye drops by HPLC
Journal Title: Eye Science (Yanke Xuebao) - Year 2024, Vol 39, Issue 12
Abstract
[Objective:] To establish a high-performance liquid chromatography (HPLC) method for the determination of content of Travoprost in Travoprost Eye Drops. [Methods:] The analytic column was Dikma C18 (50 mm×4.6 mm, 3 µm) . Using phosphoric acid solution (take 1.0 mL of phosphoric acid, dilute with water and make up to 1 000 mL, adjust the pH to 2.8 with 1 mol/L sodium hydroxide solution)-acetonitrile (67:33) as mobile phase. The flow rate is 3.0 mL/min. The column temperature is 25 ℃; The detection wavelength is 220 nm. The injection volume is 30 μL. [Results:] The linear range of travoprost showed were well shown within 20.28-70.98 μg/mL(r=0.998). The average recovery rate of travoprost was 100.3% with relative standard deviation (RSD) 2.0% (n=9). The method had high reproducibility. The reference solution and the test solution remain stable at room temperature for 48 hours. [Conclusion:] The method can be used for the determination of content of travoprost in Travoprost Eye Drops.
Authors and Affiliations
Jieting CHEN, Yujun LI, Shaomin WENG
Keywords
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- EP ID EP761496
- DOI 10.12419/24093001
- Views 24
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