Determination of efficacy, adverse drug reactions and cost effectiveness of three triple drug regimens for the treatment of Helicobacter pylori infected acid peptic disease patients
Journal Title: Asian Pacific Journal of Tropical Disease - Year 2012, Vol 2, Issue 0
Abstract
Objective: To evaluate the clinical outcome and Helicobacter pylori (H. pylori) infection status by GLQI and PCR, before and after the triple (CMO, CAO or LTC) treatment regimen. Methods: Salivary samples of 225 patients of acid peptic disease were used to determine the infection status and GLQI score before and after treatment regimen. Information regarding direct/indirect costs and side effects were determined using validated questionnaires. Results: Infection status was determined by successful amplification of 16s r RNA and Cag T genes. Positive eradication rate was 72.7% in males and 70% in females in CMO treated, 88.13% in males and 87.5% in females in CAO treated and 84.44% in males and 96.67% in females in LTC treated patients. The highest improvement in the GIQOL score after the treatment regimen was visible in the LTC treated group with a change of 65.39, followed by CAO treated patients with a change of 49.73 and CMO treated patients with an improvement of 32.18. The average cost effectiveness ratio was found to be best in the CAO treatment regimen with a ratio of 9.43 followed by LTC treatment regimen with a ratio of 11.74 and CMO with a ratio of 49.13. Side effects like diarrhea, nausea, bad taste and metallic taste were significantly enhanced in CMO (P< 0.001) when compared to CAO and LTC treatment regimens. Conclusions: The present investigation suggests that LTC is efficacious and bears less side effects, but CAO is most cost effective amongst the three treatment regimens. PCR assay can be scaled up for hospitals or clinics as a cost effective non invasive diagnostic test.
Characterization of immunogenic Clonorchis sinensis protein fractions by gel fitration chromatography
Objective: To characterize immunogenic protein fraction of Clonorchis sinensis (C. sinensis) by partial purification. Methods: A total of 30 hamsters were infected with 50 C. sinensis metacercariae, and then C. sinensis...
Single nucleotide polymorphism and expression studies of the interferon gamma gene and its role against Haemonchus contortus in Garole and Sahabadi sheep
Objective: To evaluate the role of interferon gamma (IFN-γ) gene in Haemonchus contortus (H. contortus) resistant in Garole/Sahabadi breed of sheep. Methods: Five Garole sheep (Group I) consistently showing low egg per g...
In vitro antibacterial potential of Hydrocotyle javanica Thunb.
Objective: To explore the in vitro antimicrobial potential of Hydrocotyle javanica (H. javanica) Thunb. against human pathogenic bacteria and to standardize purification protocol to harvest the active principle in large...
Studies on biochemical and biomedical properties of Conus betulinus venom
Objective: To study the biochemical and biomedical properties of predatory gastropod Conus betulinus venom. Methods: Molecular weight of the crude venom extract was determined by using SDS-PAGE. Toxicity studies were car...
Evaluation of IL-22 polymorphism in patients with visceral leishmaniasis
Objective: To examine the IL-22 gene polymorphism in visceral leishmaniasis (VL) patients in comparison to healthy people as the control group. Methods: The blood samples were collected from 236 people, among them 74 wer...