Determination of Gemifloxacin in pure and pharmaceutical forms : A spectrophotometric study
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2018, Vol 8, Issue 4
Abstract
Four simple and sensitive extractive spectrophotometric methods for the determination of Gemifloxacin in pure form and in pharmaceutical formulations using triphenyl methane dyes have been developed. The developed methods involve formation of coloured chloroform extractable ion-pair complexes of the drug with triphenyl methane dyes viz., bromothymol blue (BTB), bromophenol blue (BPB), bromocresol green (BCG) and bromocresol purple (BCP) in acidic medium. The extracted complexes formed with BTB, BPB, BCG and BCP showed absorbance maxima at 415, 416, 412 and 414 nm respectively. The stoichiometry of the ion-pair complex of Gemifloxacin with dyes is found to be 1:1 in each case. Beer’s law is obeyed in the concentration range 2.5-25µg/ml of drug with dyes. The effect of concentration of dye, pH, and interference of excipients has been studied for optimization. The limits of detection and quantification have been determined for all the four methods. These methods have been validated as per the guidelines of ICH. The results of analysis were validated statistically through recovery studies.
Authors and Affiliations
Sayanna1 . , T. Veeraiah, Ch. Venkata Ramana Reddy2
Keywords
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