Determination of LD50 and ED50 by dose response relationship and assessment of toxicological and non toxicological behaviour of Ipomoea hederacea
Journal Title: Journal of Pharmacy Research - Year 2011, Vol 4, Issue 4
Abstract
The present study was designed to evaluate the toxic and non toxic profile of Ipomoea hederacea (Convolvulaceae). The results of oral toxicity study was observed in mice at the doses of 300, 200, 100, 50, 25, 10, 1, 0.025 and 0.0125 mg/kg. The starting of lethal effect was recorded at the dose of 300 mg/kg and the calculated LD50 value was found 229.2 mg/kg dose. The toxic sign and symptoms after oral dose introduction in mice were convulsion and tremor. Dose dependent toxic effect in behaviour of mice included convulsion, tremor, unsteady gait, and respiratory distress to death. It was also observed that at the dose of 1, 0.025, 0.0125 no sign of toxicity in behaviour of animal was observed and the calculated ED50 was found 0.0125-1mg/kg. In analgesic activity test at low dose (1, 0.025, 0.0125 mg/kg) significant reduction in number of writhes were observed (p< 0.05). Hot plate analgesic activity was also exhibited some analgesic potential. Other studies like open field, exploratory behaviour, forced swimming test for stress and insecticidal activity were also carried out on the crude methanolic extract of this plant.
Authors and Affiliations
Mansoor Ahmad1*, Mehjabeen2, M. Zia-Ul-Haq1, Noor Jahan3
Keywords
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