“Determination of potential virulence factors in clinical isolates of various Candida species to assess its clinical implication in a tertiary care hospital in Eastern India”.
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2018, Vol 17, Issue 7
Abstract
Candidiasis is the commonest fungal disease in human caused by various species of the yeast like fungi belonging to the genus Candida. It is commonly encountered as an endogenous infection due to its commensal nature. Transition from commensal to a pathogen is attributable to complex array of different virulence factors which directly interacts and damages mammalian host cells. Aim: The present study was intended to determine different virulence factors expressed by clinical isolates of Candida species and to consider its clinical implication which will help in providing rational and justified therapy to the patients. Methods and Material: 240 clinical isolates of Candida species obtained from various clinical specimens like nail (86), blood (44), deep tracheal aspirate (DTA) & bronchoalveolar lavage (BAL) (28), oral swabs & buccal washings (36), urine (30), corneal ulcer (10) and ear discharge (06) were included in the study and processed for speciation following standard protocol. The virulence factors detected were phospholipase activity in egg yolk agar, haemolysin production in Sabouraud’s Dextrose Agar (SDA) supplemented with blood, esterase activity in Tween 80 media, adherence assay and production of biofilm. Results: Among 240 Candida isolates, 80 were C.albicans, 60 C.tropicalis, 44 C.parapsilosis, 24 C.lusitanae, 16 C.glabrata, 12 C.krusei, and 04(four) C.stellatoidea. 118 (49.1%) isolates showed haemolysin production, 106 (44.1%) expressed phospholipase, 76 (31.6%) expressed esterase, 94 (39.1%) strains gave positive result in adherence assay and 116 (48.3%) Candida isolates were found to be biofilm producers. Conclusions: The above study emphasizes on detecting virulence traits of Candida isolates obtained from clinical samples as contaminant commensal Candida strains are not infrequent isolation from clinical samples.
Authors and Affiliations
Dr. Reena Ray (Ghosh), Dr. Mallika Ghosh, Dr. Nibedita Chatterjee, Prof. (Dr,) Mitali Chatterjee
Keywords
Related Articles
- EP ID EP377432
- DOI 10.9790/0853-1707095661.
- Views 69
- Downloads 0
PancytopénieProfonde Révélant Une Leucémie A Burkitt : APropos D’un Seul Cas Profound Pancytopenia Revealing Burkitt’sLeukemia: A Case Report
Acute lymphoblastic leukemia (ALL) is the most common hematological malignancy in children. L3ALL or Burkitt’sleukemia is less common; its clinical manifestations that lead to the diagnosis usually reflect the medullary...
Delirium in Hospitalised Patients A Study Of Clinical and Psychosocial Profiles
Background: Very few studies from India studied clinical & psychosocial factors affecting delirium and its symptomatology using DRSR98, associated etiologies, clinical outcomes. Method: 50 consecutive referrals of deliri...
Comparative evaluation of Nickel-Titanium closed coil spring and Elastomeric chain for canine retraction. A Randomized Clinical Trial
Introduction:Preadjusted Edgewise appliance (MBT) has popularized the use of sliding mechanics, which has become the most commonly used method of achieving space closure in orthodontics. In the quest for the most efficie...
Primary breast angiosarcoma: Prognostic and therapeutic approach
Background: Primary breast angiosarcoma is a rare disease with an aggressive pattern and poor prognosis. Methods: We retrospectively reviewed clinical, pathological and therapeutic data of 10 cases of primary breast angi...
Maternal &fetal outcome of spinal v/s general anaesthesia in pre eclampsia cases undergoing caesarean section – An eyeopener !
Introduction: Women with pre-eclampsia have an increased rate of cesarean section consequent upon the high incidence of intrauterine growth restriction, fetal distress and prematurity. Cesarean section on the other hand...