Determination of tetracaine hydrochloride and ethyl hydrobenzoate in tetracaine hydrochloride eye drops by HPLC
Journal Title: Eye Science (Yanke Xuebao) - Year 2022, Vol 37, Issue 4
Abstract
[Objective:] To establish a high-performance liquid chromatography (HPLC) method for the determination of tetracaine hydrochloride and ethyl hydrobenzoate in tetracaine hydrochloride eye drops. [Methods:] The analytic column was Agilent Eclipse Plus C18 (4.6 mm×250 mm, 5 µm) and the mobile phase was 1% triethylamine solution (10 mL triethylamine and 990 mL water, pH adjusted to 5.0±0.5 with glacial acetic acid) - acetonitrile (65: 35) at a flow rate of 1.0 mL/min. The detection wavelength was 256 nm and the column temperature was 30 ℃. The injection volume was 20 μL. [Results:] The linear range of tetracaine hydrochloride was well shown within 0.05–0.36 mg/mL (r=1.000). The average recovery rate of tetracaine hydrochloride was 99.2% with relative standard deviation (RSD) 0.3% (n=9). The linear range of ethyl hydrobenzoate was well shown within 3.02–24.14 μg/mL (r=1.000). The average recovery rate of tetracaine hydrochloride was 98.2% with RSD 0.4%(n=9). The method had high reproducibility. The reference solution and testing solution were stable for 24 h in room. [Conclusion:] The method is simple, quick and accurate, which is suitable for the determination of tetracaine hydrochloride and ethyl hydrobenzoate in tetracaine hydrochloride eye drops.
Authors and Affiliations
Yujun LI, Jieting CHEN, Yingxian HU, Shumei MA, Yandong WANG
Keywords
Related Articles
- EP ID EP763653
- DOI 10.3978/j.issn.1000-4432.2022.02.05
- Views 8
- Downloads 0
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