Development and validation of a HPTLC method for simultaneous estimation of efavirenz and lamivudine in pharmaceutical formulations
Journal Title: World Journal of Pharmaceutical Sciences - Year 2016, Vol 4, Issue 2
Abstract
A simple, sensitive and rapid high performance thin layer chromatographic method has been developed and validated for the simultaneous estimation of efavirenz and lamivudine in pharmaceutical formulations. The chromatographic development was carried out on HPTLC plates pre-coated with silica gel 60G F254 using a mixture of ethyl acetate: methanol: formic acid in the ratio of 7.0:2.5:0.5 (v/v) as mobile phase. The calibration curve was found to be linear over the concentration range of 50-200 ng/spot for 3TC and 100-400 ng/spot for EFV with a regression coefficient for both analytes were greater than 0.999. The %RSD values for intra-day and inter-day variation were not more than 2.0. The method has demonstrated high sensitivity and specificity. The method is new, simple and economic for routine estimation of efavirenz and lamivudine in bulk, pre-formulation studies and pharmaceutical formulation to help the industries as well as researchers for their sensitive determination of efavirenz and lamivudine rapidly at low cost in routine analysis.
Authors and Affiliations
Madhusudhanareddy Induri
Keywords
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