Development and Validation of First Derivative Spectrophotometric Method for Quantification of Darunavir in Tablets
Journal Title: Journal of Pharmaceutical Research International - Year 2014, Vol 4, Issue 6
Abstract
Aims: Darunavir is widely used in HIV/AIDS therapy. It is a HIV protease inhibitor that has excellent efficacy against the virus. The aim of this study is to develop and validate an analytical method fast and free of interferences for determination of darunavir ethanolate as raw material and tablet dosage form. Methodology: As the formulation excipients show high interference in darunavir determination by a direct UV absorption measurement a derivative spectrophotometry was applied. A selective, easy and fast method was achieved employing simple and cheap instrumentation by using first-order derivative spectrophotometry. Results: The first-derivation of spectrum of the drug measured between 200 and 400 nm allowed identification of the analyte and showed absence of placebo interference. The assay was based on the absorbance at 276nm. The linear concentration range was established from 11 to 21 μg/mL. The intra-day and inter-day precision expressed as RSD was 0.06% and 3.75% respectively with mean recovery of 99.84%. Conclusion: The proposed analytical method is able to quantify darunavir as raw material and tablets and can be used routinely by any laboratory applying a spectrophotometer with a derivative accessory. The great difference of the method proposed here is that it proves to be free of placebo interferences as well as simple, fast and low cost.
Authors and Affiliations
Josilene Chaves Ruela Corrêa, Cristina Helena dos Reis Serra
Keywords
Related Articles
- EP ID EP344906
- DOI 10.9734/BJPR/2014/6326
- Views 122
- Downloads 0
In vitro Proliferation and Wound Healing Effects of Narcissus tazetta L. Bulb on Primary Human Dermal Fibroblasts
Aims: Narcissus tazetta L. bulbs have been used in Traditional Persian medicine (TPM), as "Zaroor" for wound healing. Toward quality assurance of the plant, pharmacognostic and phytochemical studies, physicochemical char...
Antibacterial Activity of Pennisetum glaucum Crude Extracts and Pre-purified Fractions against Selected Pathogenic Microorganisms
Background: Penisetum glaucum is one of the earliest indigenous food crops in East Africa. Pearl millet contains numerous phytocompounds. Isolation and characterization of secondary metabolites from pearl millet and eval...
Effect of Delphinium staphisagria in Murine Infection by Myocoptes musculinus
Biomedical investigations still rely on the use of laboratory animals, mice and rats are the most commonly used on experimentations. Past experimentations with these animals showed that they could be affected by environm...
Role of Magnesium Supplement on Hyperlipidemia and L-CAT Level in Patient on Atorvastatin Therapy
Aim: This study aimed to evaluate the effect of magnesium supplement to atorvastatin on hyperlipidemic patients and to elucidate the possible ability of oral magnesium supplement to counteract or delay statins induced m...
Comparative Hepatic Effects of Hippocratea africana Root Bark Extract on Female and Male Albino Wistar Rats
Aim: To compare the effects of Hippocretea africana root bark extract on liver function biomarkers of female and male albino Wistar rats. Study Design: 48 albino Wistar rats weighing 163-227 gms consisting 24 females an...