Development and validation of hplc method for simultaneous estimation of meclizine and folic acid in bulk drug and pharmaceutical formulations
Journal Title: INTERNATIONAL JOURNAL OF CURRENT RESEARCH - Year 2017, Vol 9, Issue 6
Abstract
A simple, specific, accurate, precise stability indicating reversed-phase high-performance liquid chromatographic (RP-HPLC) method was developed and validated for the simultaneous determination of folic acid (FA) and meclizine hydrochloride (MEH). An isocratic separation of FA and MEH were achieved on C 18, 250 × 4.6mm ID, 5 𝜇m particle size columns at column oven temperature 370C with a flow rate of 0.5mLmin−1 and using a diode array detector to monitor the detection at 254 nm. The mobile phase consisted of buffer: acetonitrile : trifluoro acetic acid at a ratio of 30 : 70 : 0.1 (v/v). The retention times of FA and MEH was found to be 5.25 and 10.14 min, respectively. Suitability, specificity, linearity, accuracy, precision, stability, and sensitivity of this method for the quantitative determination. The proposed method is reliable and robust and can be used as quality control tool for the estimation of these drugs in combined pharmaceutical solid dosage forms.
Authors and Affiliations
Naveen Kumar, G. S. , Srinivasa, U.
Keywords
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