Development and Validation of HPTLC Method for the Simultaneous Estimation of Telmisartan and Ramipril in Combined Dosage Form
Journal Title: International Journal of Pharmaceutical and Biological Research - Year 2010, Vol 1, Issue 1
Abstract
This paper describes developed and validated thin layer liquid chromatography (TLC) method for the simultaneous estimation of telmisartan and ramipril in a combined dosage form. Procedure does not require prior separation of components from the sample. Telmisartan and Ramipril were determined by High Performance Thin Layer chromatography method (HPTLC) in tablet dosage form. The method was carried out in TLC Precoated silica gel on aluminum plate 60 F 254, (10 cm ×10 cm, prewashed by methanol and activated at 60° C for 5 min prior to chromatography). The solvent system was Acetone: Benzene: Ethyl acetate: Glacial acetic acid in the proportion of 5:3:2:0.03, (v/v/v/v) with Rf Value for telmisartan and ramipril was 0.673 and 0.353 respectively. The linearity regression analysis for calibration showed 0.996 and 0.998 for telmisartan and ramipril with respect to peak area and height in the concentration range of 100- 1800 ng/spot and 300-1800 ng/spot respectively. The method developed can be used for routine analysis of drugs content in tablet dosage form.
Authors and Affiliations
V. A. Patel , P. G. Patel*, B. G. Chaudhary, N. B. Rajgor , S. G. Rathi2
Keywords
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