DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR ESTIMATION OF TELMISARTAN IN BULK AND FORMULATION USING FLUORESCENCE DETECTOR.
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2014, Vol 3, Issue 2
Abstract
Asensitive, accurate and precise RP-HPLC method using Fluorescence detector has been developed and validated for the estimation of TELMISARTAN from bulk drug and Pharmaceutical Dosage form. The separation was achieved by a Prontonsil ODS analytical C18 column (250mm X 4.6mm, 5μm) in isocratic mode, with mobile phase comprises of Acetonitrile : Buffer in proportion of 90:10v/v, buffer was Phosphate Buffer (pH 2.4 adjusted with Ortho Phosphoric Acid). The flow rate of mobile phase was 1.0ml/min and employing fluorescence detection with 259 nm excitation and 399 nm emission wavelengths. The retention time of TELMISARTAN was 2.6min. The calibration curve was found to be linear within the concentration range of 10ng/ml to 90ng/ml. The regression data for calibration curve shows good linear relationship with r2 = 0.9981. The method was validated in accordance with the requirements of ICH guidelines. Moreover, the proposed analytical method was applied to monitor the formulation commercially available.
Authors and Affiliations
Bhavna. A. Patel*| P.G. Department of Pharmaceutical Sciences, Sardar Patel University, Vallabh Vidyanagar, Gujarat, India., Aanandkumari. D. Captain| A.R. and G.H. Patel College of Pharmacy, Vallabh Vidyanagar, Gujarat, India.
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