DEVELOPMENT AND VALIDATION OF RP- HPLC SIMULTANEOUS ESTIMATION METHOD OF MONTELUKAST AND BAMBUTEROL FOR STABILITY INDICATING ASSAY
Journal Title: Journal of Drug Discovery and Therapeutics - Year 2013, Vol 1, Issue 9
Abstract
The objective of the current study was to develop and validate a simple, accurate, precise and selective stabilityindicating gradient reverse phase high performance liquid chromatographic method for simultaneous estimation of Montelukast (MTK) and Bambuterol (BBL) in pharmaceutical formulation in presence of degradation products. The chromatographic separation of MTK and BBL was achieved on Shimadzu LC-20AT series HPLC having C18- ODS bonded column (250 ×4.6 mm, 40 °C, 10 μL) using UV/Visible detector at 231 nm. The optimized mobile phase was consisted of a acetonitrile (100%) at a flow rate of 1.5 mL/min. The retention times were 4.65 and 2.69 min for bambuterol and montelukast respectively. The proposed method provided linear responses within the concentration ranges 0-50μg/mL for bambuterol and montelukast. The limit of detection (LOD) and limit of quantification (LOQ) values were found to be 0.0259, 0.078 μg/mL and 0.0206, 0.062 μg/mL for bambuterol and montelukast, respectively. The developed method was validated as per ICH guidelines with respect to specificity, linearity, accuracy, precision, robustness and ruggedness. The studies data revealed that developed method was convenient, fairly reliable, sensitive, less expensive and reproducible.
Authors and Affiliations
Ashish Kumar Jain*| Adina Institute of Pharmaceutical Sciences, Sagar, 470002, (M.P), India., Nivrati Jain| Adina Institute of Pharmaceutical Sciences, Sagar, 470002, (M.P), India., Harshita Jain| Adina Institute of Pharmaceutical Sciences, Sagar, 470002, (M.P), India., Prateek Kumar Jain| Adina Institute of Pharmaceutical Sciences, Sagar, 470002, (M.P), India.
Keywords
Related Articles
- EP ID EP1447
- DOI -
- Views 574
- Downloads 28
"India's progress towards Ecopharmacovigilance"
Ecopharmacovigilance is the science and activities associated with the detection, evaluation, understanding, and prevention of adverse effects of pharmaceuticals in the environment. The adverse effect of medicinal wastes...
A review on paper chromatography
Chromatography is a meaning of separating mixture by distribution of the components between stationary phase and a mobile phase .Chromatography is a method used by scientists for separating organic and inorganic compound...
HOLISTIC SUPPLEMENT OMEGA FATTY ACIDS: A CHALLENGE FOR SMART HEART BY SAYING NO TO ATHEROSCELEROSIS AND YES TO HOMEOSTASIS
Omega-3/5/6/7/9 fatty acids are considered as essential fatty acids. They are necessary for human health but the body can’t make them -- you have to get them through food. Omega fatty acids can be found in fish, such as...
INVESTIGATION OF IN VITRO ANTHELMINTIC ACTIVITY OF FICUS ELASTICA LEAVES
The World Health Organization estimates that a staggering two billion people harbor parasitic worm infections. The increasing of anthelmintic drug resistance and the high cost of anthelmintic drugs led to the development...
EVALUATION OF SEED OIL OF ZANTHOXYLUM ARMATUMFOR ANTIMICROBIAL ACTIVITY
Essential oils have proved to possess valuable antimicrobial and antimicrobial activity. The present work aims at finding out potential of essential oils whose antimicrobial activities have not been fully explored. The r...