DEVELOPMENT AND VALIDATION OF SIMPLE UV SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF SELEXIPAG IN API AND ITS BULK DOSAGE FORM
Journal Title: Indo American Journal of Pharmaceutical Research - Year 2017, Vol 7, Issue
Abstract
Abstract The present study was undertaken to develop and validate a simple, accurate, precise, reproducible and cost effective UV-Visible spectrophotometric method for the estimation of Selexipag. The solvent used throughout the experiment was Dimethyl sulphoxide (DMSO). Absorption maximum (λmax) of the drug was found to be 306 nm. The quantitative determination of the drug was carried out at 306 nm and Beer’s law was obeyed in the range of 5-25μg/mL. The approach of this work includes preliminary literature survey followed by the practical method development, applicable to be used on regular basis. The major outcomes of this method includes following: method was shown linear in the mentioned concentrations having line equation y =0.045x-0.039 with correlation coefficient R2 of 0.9963. The recovery values of Selexipag for 80%, 100% and 120% were found to be 99.16%, 99.7% and 99.00% respectively. The percent relative standard deviation (RSD %) of interday precision was 0.490% and intraday precision was 0.28%. The limit of detection and limit of quantification was 0.477μg/mL and 1.44μg/mL. The percent relative standard deviation of robustness and ruggedness of the method was 1.78 and 0.38% respectively. It can be concluded that proposed method was precise, accurate and cost effective and it could be applicable for quantitative determination of the bulk drug as well as dosage formulation.
Authors and Affiliations
Kotwal T. S*, Patwardhan D. M. , Amrutkar S. S. , Wagh M. P.
Keywords
Related Articles
- EP ID EP330793
- DOI -
- Views 103
- Downloads 0
RECENT PROSPECTIVE ON SOLID LIPID NANOPARTICLES AND ITS APPLICATION- A REVIEW
Abstract Solid Lipid Nanoparticles are at the forefront of the rapidly developing field of nanotechnology with several potential applications in drug delivery and research. Due to their unique size dependence properties,...
EXPLORATION OF VARIOUS CLASSICAL AND CHEMOMETRIC ASSISTED UV SPECTROPHOTOMETRIC METHODS FOR ESTIMATION OF CHLORHEXIDINE GLUCONATE AND CETRIMIDE IN BULK AND ITS FORMULATION
Abstract The present work inculcates with the approach for development of various classical and chemometrics assisted UV spectrophotometric methods for estimation of Chlorhexidine gluconate (CH) and Cetrimide (CET) in bu...
SIO2.CAA: AN EFFICIENT CATALYST FOR ONE POT SYNTHESIS OF 4,6- DIARYLPYRIMIDINE- 2(1H)-ONES OR THIONES
Abstract An efficient method for the synthesis of 4,6-diarylpyrimidin-2(1H)-ones or thiones by using SiO2.CAA. The condensation of acetophenone, aldehydes and urea or thiourea in the presence of silica supported catalyst...
PLIGHTS AND PREDICAMENTS IN THE PHARMACY INDUSTRY
We provide an insight to the troubles in the field of pharmacy. Using the published literature to date, we review the impact of various challenges in the field of Pharmacy. The pharmaceutical industry has succumbed to th...
Formulation and Evaluation of Amlodipine besylate orally disintegrating tablet
Amlodipine besylate is a recognized drug for hypertension therefore development of an ODT of Amlodipine besylate and to evaluate the effect of various superdisintegrants on its disintegration time and release profile was...