DEVELOPMENT AND VALIDATION OF UV SPECTROSCOPIC METHOD FOR THE ESTIMATION OF AQUEOUS EXTRACT OF FAGONIA SCHWEINFURTHII HADIDI
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2014, Vol 5, Issue 2
Abstract
A simple, rapid, accurate, precise, and economic spectrophotometric method for aqueous extract of Fagonia schweinfurthii hadidi have been developed. Aqueous extract of plant was obtained from decoction process of coarse powder of whole plant. Aqueous extract of plant shows absorbance maximum at 265 nm when Phosphate Buffer of pH 6.8 and 0.1N HCl used as solvent system. Calibration curves were constructed in phosphate buffer pH 6.8 and 0.1 N HCl. Beer’s law was obeyed in the concentration range of 25-350 µg/ml. In phosphate buffer pH 6.8 the equations and R2 obtained, were y = 0.0025x - 0.0083 and R2 = 0.9995 respectively and in 0.1N HCl the equations and R2 obtained were y = 0.0024x + 0.0181 and R2 = 0.9987 respectively. Validation of developed method was done according to I.C.H. guideline.
Authors and Affiliations
Dinesh Puri, Anil Bhandari, Shailendra Sharma, Sanjay Sharma, Rambabu Sharma, Deepak Choudhary
Keywords
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- EP ID EP126183
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