Development of microsatellite markers and their correlation with morphological and chemical markers in [i]Withania somnifera [/i](L.) Dunal
Journal Title: Journal of BioScience and Biotechnology - Year 2016, Vol 5, Issue 1
Abstract
The genetic variation and relationships among 14 [i]Withania [/i]accessions were evaluated using morphological, chemical and Simple Sequence Repeat (SSR) markers. Wild accessions are more robust and better performing in morphological and chemical metabolite accumulation than cultivated one. The results revealed that out of fourteen, four primers showed distinct polymorphism, indicating the robust nature of microsatellites in revealing polymorphism. The banding pattern was recorded in the form of 0-1 data sheet which was analyzed using unweighted pair group method with arithmetic mean (UPGMA) based on Jaccard's similarity coefficient. The cluster analysis showed higher level of genetic variation among the accessions. Similarity coefficients ranged from 0.125 to 1. The dendrogram revealed 3 major distinct clusters. Higher range of similarity values for related genotypes using simple sequence repeats (SSR) provides greater confidence for the assessment of genetic diversity and relationships. The polymorphism information content (PIC) value for the SSR loci ranged from 0.0 to 0.40. Higher PIC values were associated with higher level of polymorphism. Results of this study showed a high degree of variation among analyzed accessions, indicating an important source of genetic diversity that can be used in future breeding programs.
Authors and Affiliations
Punit Khanna, Ratna Chandra, Arun Kumar, Nidhi Dogra, Heena Gupta, Gourav Gupta, Vijeshwar Verma
Keywords
Related Articles
- EP ID EP117813
- DOI -
- Views 88
- Downloads 0
Molecular cloning, expression and in vitro analysis of soluble cationic synthetic antimicrobial peptide from salt-inducible E. coliGJ1158
Antimicrobial peptides are the upcoming therapeutic molecules as alternative drugs to the existing antibiotics owing to their potent action against pathogenic microorganisms. In this study, to obtain an antimicrobial pep...
Analyzing inhibition of BCL11A gene expression in K562 cells by RNAi
RNA interference (RNAi), an effective approach to sequence-specific gene knockdown is widely used for the investigation of regulation of gene expression in various cells. BCL11A (B cell lymphoma 11A) plays a vital role i...
Bacterial interference with host epithelial junctional complexes: Probiotic bacteria vs. A/E lesion-forming Escherichia coli
During colonization, enteropathogenic (EPEC) and enterohaemorrhagic (EHEC) Escherichia coli are capable to manipulate host cytoskeleton and colonize gut epithelia by a specific mode of attachment known as the attaching a...
Comparison between Corchorus olitorius and Corchorus capsularis at GUS histochemical assay performance for tissue culture independent transformation
Tissue culture independent transformation technique in crops is relatively new and of popular interest due to its faster approach and efficiency. The prospect of this technique in the production of transgenic Jute...
Qualitative and quantitative determination of protopine in Fumaria spp. by TLC-densitometry method
A rapid and accurate TLC-densitometry method for qualitative and quantitative determination of protopine has been developed. The best separation was achieved using a mobile phase chloroform – ethyl acetate – methanol – a...