DEVELOPMENT OF MOLECULAR IMPRINTED POLYMER SOLID PHASE EXTRACTION (MISPE) FOR SEPARATION NITROFURANTOIN RESIDUE IN CHICKEN EGGS
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2017, Vol 10, Issue 6
Abstract
Background: The use of nitrofurantoin and other nitrofuran antibiotics in food which produced from animals is prohibited by European Union because of potentially carcinogenic and mutagenic. Various methods for analysis of residues of nitrofurantoin has been developed, but because of the interference of the matrix, it is necessary to separate the matrix therefore, the matrix effect will not interfere the analysis. Nowadays, molecular imprinted polymer (MIP) is a well-developed tool in the analytical field, mainly for separating substances in relatively complex matrices.Objective: The purpose of this study is to obtain MISPE that is selective for the separation of nitrofurantoin residues in chicken eggs.Methods: Analytical methods development of nitrofurantoin were optimization of HPLC system and validation of analytical methods performed to obtain the suitable system for nitrofurantoin detection. In silico study used for MIP design by observing the difference Gibbs free energy using Gaussview 5.08 software with Density Functional Theory (DFT) methods using 6-311G as basis set. MIP synthesis was done using bulk method use nitrofurantoin as template, acrylamide as functional monomer, ethyleneglycoldimethacrylate (EGDMA) as crosslinker, and azobisisobutyronitrile (AIBN) as an initiator reaction inside dimethylformamide (DMF) as solvent. Non imprinted polymer (NIP) was synthesized as comparison. MIP and NIP which has been synthesized was inserted into SPE cartridge and characterized using Infrared spectroscopy and HPLC.Result: MISPE that has been synthesized was characterized and compared to non-imprinted polymer solid phase extraction (NISPE) and marketed Solid Phase Extraction (SPE) C18. Sensitivity of MIP, NIP, and SPE C-18 to nitrofurantoin was 84.54 %, 37.73 %, and 33.95 % respectively, based on recovery of nitrofurantoin.Conclusion: Based on the result it was obtained MISPE has high selectivity toward nitrofurantoin compared to NISPE and either marketed SPE. Â
Authors and Affiliations
Sophi Damayanti, Untung Gunawan, Slamet Ibrahim
Keywords
Related Articles
- EP ID EP605308
- DOI 10.22159/ajpcr.2017.v10i6.17109
- Views 121
- Downloads 0
ACACIA SENEGAL BARK EXTRACT IN REGULATION OF HYPOTHYROIDISM: AN EXPERIMENTAL APPROACH
Objective: The objective of the present study was to evaluate the thyroid hormone stimulating efficacy of Acacia senegal (Gum Arabic) bark extract in 6-n-propyl-2-thiouracil (PTU)-induced hypothyroid albino rats.Methods:...
TO STUDY THE DRUG USE PATTERN IN PRIMARY OPEN ANGLE GLAUCOMA IN A TERTIARY CARE TEACHING
Objective: To study the drug utilization pattern in patients suffering from primary open-angle glaucoma (POAG) in a tertiary care teaching hospital.Methods: It is a prospective study done on 50 patients of POAG (comprisi...
SIDE EFFECTS OF INTRAVENOUS GANCICLOVIR AND COMBINATION OF INTRAVENOUS GANCICLOVIR-ORAL VALGANCICLOVIR IN PEDIATRIC PATIENTS WITH CYTOMEGALOVIRUS INFECTION
Objective: Intravenous ganciclovir and oral valganciclovir are known to have severe side effects against hematopoiesis and kidney function. This study aims to look for these side effects in pediatric patients with cytome...
ANALYSIS OF THE ANTIOXIDANT PROPERTY, CYTOTOXICITY AND ANTI-TUMOUR EFFICIENCY OF BAUHINIA PHOENICEA.
Objective: Bauhinia phoenicea Wight and Arn. is a medicinal plant endemic to Southern Western Ghats. In the traditional systems of medicine, it is using against various ailments including some oxidative disorders. Detail...
TRADITIONAL USES, PHYTOCHEMISTRY, AND PHARMACOLOGICAL ACTIVITIES OF AMLA WITH SPECIAL REFERENCE OF UNANI MEDICINE - AN UPDATED REVIEW
Emblica officinalis, commonly known as Amla belongs to family Euphorbiaceae, is widely used for medicinal purposes in Indian traditional system of medicine (Unani, Ayurveda, and Siddha). It is well known that all parts o...