Dextran-Conjugated Anti-Immunoglobulin Antibodies: A Powerful Tool for Studying B Cell Receptor-Mediated Signaling
Journal Title: International Journal of Immunology and Immunotherapy - Year 2016, Vol 3, Issue 2
Abstract
Dextran-conjugated anti-immunoglobulin (Ig) antibodies (anti-Ig-dex) consist of a high molecular weight dextran (~1-2 × 106 MW) to which multiple monoclonal anti-IgM or anti-IgD antibodies are covalently linked. These soluble conjugates have been used in a process of discovery, in extensive studies of in vitro polyclonal murine and human B cell activation, proliferation, Ig secretion and class switching, through signaling via multivalent cross-linking of B cell receptors (BCR) on the B cell surface, in the presence of a multiplicity of added stimuli. The cross-linking of the BCR in a multivalent fashion represents a more physiologic means, relative to using unconjugated bivalent anti-Ig, for studying the functional consequences of B cell encounter with specific antigens in vivo. This reflects either the repeating epitope nature of polysaccharides, or the display of a given intact protein in multiple copies on the surface of follicular dendritic cells, macrophages and dendritic cells where they are encountered by B cells.
Authors and Affiliations
Keywords
Related Articles
- EP ID EP340826
- DOI 10.23937/2378-3672/1410022
- Views 141
- Downloads 0
Uridine Cytidine Kinase Like-1 Enhances Tumor Cell Proliferation and Mediates Protection from Natural Killer-Mediated Killing
Uridine cytidine kinase like-1 (UCKL-1) is a largely uncharacterized protein over-expressed in many tumor cells, especially in highly malignant, aggressive tumors. Sequence analysis indicates that UCKL-1 has homology to...
Visions of the Hereafter: Releasing the Brakes of the Immune System by Checkpoint Inhibition Immunotherapy
Activation and control of the immune system is regulated by costimulatory molecules as well as by checkpoint inhibitors. Checkpoints are essential in maintaining self-tolerance and minimizing collateral damage by modulat...
The Diagnostic Role of Cardiac Magnetic Resonance Imaging in Rheumatoid Arthritis
In patients with rheumatoid arthritis (RA), cardiac involvement such as myocarditis and myocardial infarction is common. This cardiac involvement may have serious consequences and can contribute to worsening of a patient...
Epstein-Barr Virus (EBV) Encoded Dutpase Exacerbates the Immune pathology of Lupus Nephritis In Vivo
Epstein-Barr virus (EBV) is an environmental factor with strong links to systemic lupus erythematous (SLE) pathogenesis; however, the mechanism(s) remains unclear. The goal of this study was to determine whether the EBV...
Dextran-Conjugated Anti-Immunoglobulin Antibodies: A Powerful Tool for Studying B Cell Receptor-Mediated Signaling
Dextran-conjugated anti-immunoglobulin (Ig) antibodies (anti-Ig-dex) consist of a high molecular weight dextran (~1-2 × 106 MW) to which multiple monoclonal anti-IgM or anti-IgD antibodies are covalently linked. These so...