Diagnosis of Urinary Tract Infection - Validity of Rapid Quantitative Unspun Urine Microscopy
Journal Title: International Journal of Contemporary Medical Research - Year 2017, Vol 4, Issue 3
Abstract
Introduction: Urinary tract infections (UTIs) are among the most common bacterial infections and account for a significant part of the workload in clinical microbiology laboratories. However, the diagnosis of UTI is not always straightforward. Urine culture is considered as gold standard for diagnosis of UTI but it is time consuming and laborious. Leucocytes in the urine demonstrate inflammation and tissue invasion of the UTI, distinguishing infection from colonization or contamination. Therefore, pyuria can be used as an indicator of bacteriuria. This retrospective study was conducted to assess the usefulness of quantitative unspun urine microscopic examination for the rapid diagnosis of urinary tract infections. Material and Methods: The present retrospective study was conducted at Guru Gobind Singh Medical College and Hospital, Faridkot. For this, clean catch, midstream urine samples were collected in sterile, wide mouthed, universal container from 2299 patients clinically suspected of UTI. The specimens were immediately transported to the laboratory and processed within 2 hours of the collection. Semi quantitative culture was done on blood agar and Mac Conkey agar and colonies were counted after overnight incubation at 37°C. Cut off for significant bacteriuria was taken as 105 cfu/ml. Quantitative uncentrifuged wet mount microscopic examination was done of well mixed uncentrifuged urine sample and a drop of which was examined under high power field of microscope for the presence of pus cells. The cut off for significant finding was ≥1pus cell /7hpfs for pyuria. Results: A total of 2299 urine samples of patients were tested. Out of which, 861 (37.5%) had pus cells on wet microscopic examination while 754 (32.8%) had growth on semi quantitative culture of urine. Correlation of microscopic examination of urine with urine culture showed Sensitivity: 92.7%, Specificity: 89.5%, PPV (Positive predictive value): 81.1%, NPV (Negative predictive value): 96.2% Conclusion: Quantitative uncentrifuged wet microscopy of urine is a rapid, reliable valuable time saving and cost effective technique which could be added into routine laboratory practice for faster diagnosis of UTIs.
Authors and Affiliations
Renu Bansal, Rajinder Paul Jindal, Rupinder Jossan
Keywords
Related Articles
- EP ID EP428653
- DOI -
- Views 124
- Downloads 0
Ultrasonography in Analyzing Appendicular Lesions based on Contents and Caliber
Introduction: Ultrasonography is equally sensitive and specific to computed tomography in diagnosing acute appendicitis with similar positive predictive value. Current study aimed to investigate the role of Ultrasonograp...
Deadly Combination; Ti̇kagrelor and Abciximab
Introduction: Incremental improvements in pharmacotherapy for percutaneous coronary intervention continue to reduce the incidence of adverse clinical events for the treatment of both stable coronary artery disease and ac...
Role of Caudal Epidural Injection in Managing Failed Back Syndrome: One Year Follow Up Result of A Randomized Study
Introduction: There is an increasing prevalence of low back pain in the modern era, which is associated with wide range of diagnostic and therapeutic modalities. The Spine Patient Outcomes Research Trial (SPORT) have dem...
Medical Management of Oral Submucous Fibrosis Our Experience
Introduction: Oral submucous fibrosis is a premalignant condition of oral cavity characterised by scaring of oral mucosa with restricted mouth opening and is caused by chewing of arecanut. The pathogenesis of the disease...
Pediatric Flexible Bronchoscopy Under Conscious Sedation in an Endoscopy Suite – Is It Feasible, Safe and Yielding?: An Observational Study
Introduction: The first description of bronchoscopy in pediatric practice was in 1978. Current study aimed to record the safety and diagnostic yield of performing fibre optic bronchoscopy (FB) in children under sedation...