Diagnostic accuracy of squash cytology and role of GFAP immunoexpression in glial tumors
Journal Title: Indian Journal of Pathology and Oncology - Year 2018, Vol 5, Issue 1
Abstract
Introduction Intraoperative squash technique provides rapid and reliable diagnosis which is helpful to monitor or modify the treatment approach We carried out the present study to compare the accuracy of squash preparation with histopathological diagnosis and also to study the role of GFAP immunostaining in gliomasMaterial and Methods Clinicoradiologically diagnosed 127 cases of gliomas were subjected to both squash smear cytology and histopathological examination also to GFAP staining using monoclonal antibody EP672Y clone Taking histopathology as gold standard diagnostic accuracy and statistical values were calculated Immunohistochemical analysis was compared with final diagnosisResults Overall diagnostic accuracy of squash cytology was observed as 952 sensitivity991 PPV 991 All low grade astrocytomas and ependymoma showed strong diffuse positivity while high grade astrocytomas were focally reactive Negative immunoexpression was seen in case of oligodendroglioma and pituitary adenomaConclusion Squash cytology is of great value in rapid intraoperative consultation of gliomas GFAP can be used as an adjunctive tool to verify the astroglial differentiation especially in diagnostically difficult casesKeywords diagnostic accuracy GFAP Gliomas Squash
Authors and Affiliations
Sanjeev Kishore, Brijesh Thakur, Aparna Bhardwaj, Anuradha Kusum
Keywords
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- EP ID EP473174
- DOI 10.18231/2394-6792.2018.0003
- Views 61
- Downloads 0
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