Diagnostic Performance of Polymerase Chain Reaction in Suspected Cases of Herpes Simplex Encephalitis

Journal Title: International Journal of Medical Research Professionals - Year 2018, Vol 4, Issue 5

Abstract

Introduction: One of the prime diagnostic goals in suspected cases of viral encephalitis is to identify a specific etiologic agent, with emphasis on disease that requires therapeutic intervention, such as Herpes simplex encephalitis. Diagnosis of viral infections of the central nervous system has been revolutionized by the advent of new molecular diagnostic technologies, such as the standard Polymerase chain reaction/Real time polymerase chain reaction. The technique can be performed rapidly and inexpensively, with a turnaround time of 24 h or less. Materials and Methods: A total of 54 patients suspected of HSV encephalitis in adult or paediatric patient with fever (≥380C), altered mental status, whether with or without headache, seizures or focal neurologic abnormalities were selected for study. The PCR reactions were optimized with the same set of primers and reaction conditions both for the known HSV control strain and all the 54 patients CSF samples. Results: Overall, 6 (11.1%) of the CSF samples were positive for HSV DNA. Negative CSF controls yielded negative results for HSV DNA, thus confirming the efficiency and efficacy of the amplification process. Conclusion: In conclusion, detection of Viral DNA in CSF by PCR for the diagnosis of suspected Herpes simplex virus encephalitis patients should clearly be the standard diagnostic modality.

Authors and Affiliations

Satish Kumar, Shiv Parshad Mittal, Sameer Singh Faujdar

Keywords

Related Articles

Eruptive Collagenomas: A Case Report and Review of Literature

Collagenoma or connective tissue nevus is a rare hamartomatous lesion that mainly consists of dermal collagen. Collagenomas are classified into hereditary and acquired collagenomas. Eruptive collagenomas are acquired col...

The Comparative Study of Efficacy and Safety of Phacoemulsification And Manual Small Incision Cataract Surgery (MSICS) at S. P. Medical College Bikaner

Introduction: In 2002, WHO estimated that blindness affected 37 million people globally. If efforts are not increased to treat avoidable blindness worldwide this is projected to increase to 76 million by the year 2020. T...

Incidence and Risk Factors for Retinopathy of Prematurity in Premature Born Children in Tabuk City

Introduction: Retinopathy of prematurity (ROP) is a disease of the eye that considers as one of a complication of preterm birth which characterized by abnormal growth of retina blood vessels at a junction of the vascular...

Ophthalmic Disorders among Children with Down Syndrome: An Insight into Parents’ Knowledge, Awareness Level and Attitude

Introduction: Down syndrome is a genetic disorder caused by the presence of an extra copy of chromosome 21. Ophthalmic disorders affecting children are even more frequent in those with DS and sometimes tend to be more se...

Curious Odyssey of Teeth Through Body Spaces: Report of a Rare Case

Foreign bodies in the liver following accidental ingestion, although rare, have been described in literature. In most of the cases, adults may swallow the foreign bodies which reach the liver by penetrating stomach, colo...

Download PDF file
  • EP ID EP536740
  • DOI 10.21276/ijmrp.2018.4.5.016
  • Views 103
  • Downloads 0

How To Cite

Satish Kumar, Shiv Parshad Mittal, Sameer Singh Faujdar (2018). Diagnostic Performance of Polymerase Chain Reaction in Suspected Cases of Herpes Simplex Encephalitis. International Journal of Medical Research Professionals, 4(5), 68-71. https://www.europub.co.uk/articles/-A-536740