Diagnostic Utility of Cell Block Method versus Cytospin Method in Pleural and Peritoneal Fluid Cytology
Journal Title: Journal of Medical Science And clinical Research - Year 2016, Vol 4, Issue 11
Abstract
Background: Cytological evaluation of body cavity fluid is diagnostically challenging. Improved ethanol formalin fixative is used which offer excellent cytomorphological features. Cell blocks prepared from residual tissue fluids or effusion obtained by aspiration, can be useful adjunct to smear for establishing a more definitive cytopathologic diagnosis. . Methods: A total of 170 fluid specimens were examined for cytospin smear and cell block method. Out of 170 fluids, 102 were peritoneal and 68 were pleural. Each fluid specimen was subjected to cytospin smear (CSS) technique, and 10% alcohol-formalin cell block (CB) technique. Overall morphological details, cellularity, architecture, nuclear and cytoplasmic details were studied in both CSS and CB techniques. Results: In this study, analysis body fluid specimens using cytospin smear and cell block methods revealed that there is no difference between cytospin smear method and CB in defining the benign, fungal and inflammatory conditions. However, CB method could able to identity papillary pattern more efficiently than the cytospin method. Conclusion: Although there was no statistical difference between the results obtained by the cytospin and cell block methods, cell block method in our study accurately diagnosed the cases which were missed or incompletely diagnosed on cytospin smear method. Thus cell block proved to be superior method for the study of effusion as compared to cytospin smear. As the cell blocks permit longer storage and additional analysis such as immunohistochemistry (IHC) and microarray, it should be adopted additionally for effusion cytology
Authors and Affiliations
Mulkalwar M
Keywords
Related Articles
- EP ID EP214761
- DOI -
- Views 77
- Downloads 0
Morphological Typing of Extranodal Lymphoma with Immunohistochemical Study
Background: Lymphomas are neoplasms of the lymphoid tissue. It can affect the lymph nodes as well as extra-nodal tissue. A substantial percentage of non-Hodgkin's lymphomas arises from tissue other than lymph nodes. Thes...
Comparison of Difficult Intubation and Neck Circumference to Thyromental Distance Ratio, in Obese and Non-Obese: A Clinical Study
Background: This study was conducted to assess whether intubation is more difficult in obese patients compared to non-obese and to assess the ability of ratio of the neck circumference to thyromental distance (NC/TM), to...
A Study of Urea, Uric Acid and Creatinine Pathogenesis of Oral Submucous Fibrosis
Oral Submucous Fibrosis (OSMF) is a high risk precancerous condition, chronic debilitating disease of the oral cavity characterized by inflammation and preogressive fibrosis of the submucosal tissues (Lamina Propariaand...
Could Migrain be A Part of History?
As what is called migrain hasn't any definitive aetiology [almost diagnosed by exclusion], I tried to have an explanation of that mesterious headache on the light of the basic medical sciences {anatomy. physiology. patho...
Clinical Profile of Acute Kidney Injury in Intensive Care Unit
Acute kidney injury is associated with major inpatient morbidity and mortality, reflecting the severity of the causal illness and the high frequency of complications. AKI complicates nearly 5% of the hospital admissions...