Dietary conjugated linoleic acid isomers and selenite or selenized yeast affect fatty acid concentration in the kidneys of rats
Journal Title: Journal of Animal and Feed Sciences - Year 2011, Vol 20, Issue 3
Abstract
The influence of diets enriched in a mixture of conjugated linoleic acid (CLA) isomers (CLAmix) and/or selenium as Na2 SeO3 (SeIV) or selenized yeast (SeY) on feed intake, kidneys’ weight and the concentrations of fatty acids (FA) in the kidneys of rats was investigated. The study was performed on 80 female Wistar rats (Hsd Brl Han: WIST), 8 weeks of age with an initial body weight of 195.7±0.8 g. Each group numbered 8 rats. During the 7-day preliminary period the rats were fed a standard Labofeed H diet at a sub-maintenance level. Next, for 6 weeks the rats were fed ad libitum on the experimental diets supplemented with CLAmix, 0.2 ppm Se or 0.5 ppm Se as SeIV (LSeIV or HSeIV, respectively) and SeY (LSeY or HSeY, respectively). The rats were sacrificed at the end of the six-week experimental period. The diets enriched in CLAmix or Se (as SeIV or SeY) showed a negligible effect on kidneys’ weight, although the diets containing simultaneously CLAmix and Se numerically increased kidneys’ weight compared with the control animals. The addition of CLAmix to the diet, regardless of the higher level of SeY, showed a tendency to decrease in feed intake and decreased the concentrations of saturated (SFA), mono- (MUFA) and polyunsaturated (PUFA) fatty acids in comparison with the control rats. Dietary SeIV or SeY, irrespective of the concentration of extra Se, decreased the concentration of unsaturated fatty acids (UFA) and the sum of all assayed fatty acids (ΣFA) in the kidneys. The diet containing CLAmix and Se as HSeIV or LSeY decreased the concentrations of SFA, MUFA and PUFA in the kidneys compared with the control rats. On the other hand, the addition of LSeIV or, especially, HSeY to the diet enriched in CLAmix resulted in an increasing in the concentrations of SFA, MUFA and PUFA in the kidneys compared with the control group and rats fed the diets containing CLAmix, SeIV or SeY. The diet enriched in CLAmix and HSeY most effectively stimulated the accumulation of CLA isomers with the exception of cis11trans13CLA in the kidneys in comparison with other diets. Dietary CLAmix decreased the capacity of ∆9-desaturase in the kidneys compared with the control rats. The addition of SeIV or SeY to the diet increased the capacity of ∆4-desaturase, while the diet containing CLAmix with or without Se as SeIV or SeY increased the capacity of ∆4- and ∆5-desaturase in the kidneys compared with rats fed the diet with extra Se or the control diet. The present studies documented that dietary CLAmix resulted in decreasing in biosynthesis of fatty acids in the kidneys, while the diet with CLAmix and HSeY most significantly stimulated the accumulation of SFA, MUFA and PUFA and long-chain PUFAn-3 and PUFAn-6 in the kidneys.
Authors and Affiliations
M. Czauderna, J. Kowalczyk, K. A. Krajewska, A. J. Rozbicka-Wieczorek
Keywords
Related Articles
- EP ID EP75040
- DOI -
- Views 139
- Downloads 0
Effect of Tremella fuciformis ferment substance on the growth performance and lipid metabolism of finishing pigs
An experiment was conducted to study the effects of Tremella fuciformis ferment substance (TFS) supplementation in diets on the growth performance, fat deposition, blood lipids, and mRNA expression of lipid storage-relat...
Evaluation of methods used in vivo to estimate the transfer of amino acids and other substrates into the cells of mammary gland
mino acids and other substrates into the cell in vivo using arteriovenous concentration difference and volume blood flow data with their recalculation according to kinetic model of the system: capillary - interstitium -...
Responses of mammary amino acid metabolism and aminopeptidase N gene expression to duodenal soyabean small peptides and infusion of free amino acids in lactating goats
The duodenal perfusion technique was used to determine whether small peptides were more effective than free amino acids (FAA) for milk protein synthesis and to investigate the effects of infusing soyabean small peptides...
Effects of dietary fish oil on NFκ B gene expression and related signaling in spleen of chickens stimulated with lipopolysaccharide
The study was conducted to investigate the effects of fish oil and maize oil on nuclear factor kappa B (NFκB) gene expression and the downstream pathways of intracellular signaling in spleen of chickens after lipopolysac...
Efficacy of different levels of Escherichia coli phytase in hens fed maize-soyabean meal based diets with a decreased non-phytate phosphorus content
The aim of this experiment was to determine the efficacy of different inclusion levels of new type phytase (ECP) synthesized by Escherichia coli and expressed in Pichia pastoris yeast (Optiphos) in maize-soyabean meal ba...