Differential effects of genistein, estradiol and raloxifene on rat osteoclastsin vitro.
Journal Title: Pharmacological Reports - Year 2005, Vol 57, Issue 3
Abstract
Genistein, a major phytoestrogen of soy, is considered a potential drugfor prevention and treatment of postmenopausal osteoporosis. It is not clear whether mechanism of actionof genistein on bone turnover is distinct from that of estradiol or raloxifene. The aim of the presentstudy was to investigate the effects of genistein on the formation of osteoclasts from neonatal rat bonemarrow cells in vitro, and compare them with those of estradiol and raloxifene. Formation of osteoclastswas stimulated by 1,25-dihydroxyvitamin D(3), added to the culture media on the second day after plating,together with genistein (10(-8)-10(-5) M), estradiol (10(-10)-10(-7) M) or raloxifene (10(-9)-10(-6)M). The bone marrow cell culture lasted 7 or 9 days. Number of osteoclasts and number of osteoclast "ghosts"(necrotic giant cells) were determined. Genistein, estradiol and raloxifene, at some concentrations,decreased the number of osteoclasts after 9-day culture of bone marrow cells. Genistein decreased thenumber at 10(-8) M because of decreasing the viability of osteoclasts, whereas at 10(-5) M due to attenuationof osteoclast formation. Estradiol decreased the osteoclast number at 10(-9) M due to decreasing theirviability, whereas at 10(-8) and 10(-7) M it was the effect of both decreasing the viability and inhibitionof the formation. Decreases in the number of osteoclasts caused by raloxifene (10(-9), 10(-8) M werethe effect of decreasing the viability of these cells.
Authors and Affiliations
Leszek Śliwiński, Joanna Folwarczna, Waldemar Janiec, Grzegorz Grynkiewicz, Katarzyna Kuzyk
Keywords
Related Articles
- EP ID EP101898
- DOI -
- Views 87
- Downloads 0
Effects of amiloride and bumetanide on hyperpolarization after movement across the distal colon epithelium.
The aim of the paper was to define which transepithelial ion transport pathways were activated during stimulation by movement across the colonic epithelium. The experiments were performed with Ussing method on 241 specim...
Effects of blockade of central dopamine D1 and D2 receptors on thermoregulation, metabolic rate and running performance.
To assess the effects of a blockade of central D1- and D2-dopaminergic receptors on metabolic rate, heat balance and running performance, 10 nmol (2 microl) of a solution of the D(1) antagonist SCH-23390 hydrochloride (S...
Lack of the antianxiety-like effect of (S)-3,4-DCPG, an mGlu8 receptor agonist, after central administration in rats.
Substances acting as agonists of group III mGlu receptors were shown to induce an antianxiety-like effect after intrahippocampal administration to rats. The purpose of the present study was to establish whether the selec...
Mitochondrial biogenesis in cardiac pathophysiology.
Cardiac performance depends on a fine balance between the work the heart has to perform to satisfy the needs of the body and the energy that it is able to produce. Thus, energy production by oxidative metabolism, the mai...
Immobility stress induces depression-like behavior in the forced swim test in mice: effect of magnesium and imipramine.
Previously, we demonstrated antidepressant-like effect of magnesium (Mg) in the forced swim test (FST). Moreover, the joint administration of Mg and imipramine (IMI) at ineffective doses per se, resulted in a potent redu...