Dynamic of IS6110 transposase, transframe protein responsible for IS6110 element transposition in Mycobacterium tuberculosis strains
Journal Title: Scientific Journal of Microbiology - Year 2015, Vol 5, Issue 3
Abstract
Transposition of IS6110, the Mycobacterium tuberculosis Complex specific insertion sequence, is mediated by the complex open reading frame AB (orfAB) transframe protein or transposase. Transposase has the crucial role to ensure IS6110 transposition process. A single transposase proteine can control the integration of many IS6110 copies at the same time. In this work we investigated the dynamic and the evolutionary trends of IS6110 transposase coding gene isolated from various hosts. We had subjected these sequences to several statistical tests combined with programming package analysis. Our findings showed that IS6110 transposase-encoding orfAB is subjected to purifying selection with low genetic variability rate, as demonstrated by its significantly negative Tajima’s D statistics as well as by LRT analysis and models comparison. Even some codons tended to be positively selected, global p-value didn’t reach reliable inference (ω > 1) of significant positive selection at these positions; so statistically they were not significant. In conclusion, our findings demonstrated that IS6110 transposase-encoding orfAB evolved essentially by point mutations under purifying selection acting against deleterious mutations, thus leading to an excess of low-frequency variants and purging disadvantageous non synonymous changes.
Authors and Affiliations
Sara Thabet*| Bacteriology Laboratory, Veterinary Research Institute of Tunisia, Institute for Scientific Agricultural Research, 20 rue Jabal Lakhdhar, 1006, La Rabta,Tunis, Tunisia., Nada Suissi| Bacteriology Laboratory, Veterinary Research Institute of Tunisia, Institute for Scientific Agricultural Research, 20 rue Jabal Lakhdhar, 1006, La Rabta,Tunis, Tunisia.
Keywords
Related Articles
- EP ID EP1212
- DOI 10.14196/sjm.v5i3.2157
- Views 510
- Downloads 32
Microbial diversity of oil spills and tar resistant bacteria isolated from beaches of Goa (India)
Oil contaminated samples collected from seven beaches of Goa (India) were analyzed for their microbial components. The alkaline pH, the presence of oil spill and tarball residue and high salt concentration were features...
Molecular characterization of thermophilic Streptomyces using amplified 16s ribosomal DNA restriction analysis (ARDRA)
The present study aims to characterize the 19 thermophilic Streptomyces isolates on the basis of their restriction profile obtained by Amplified Ribosomal DNA Restriction Analysis (ARDRA) method. Nineteen thermophilic St...
Screening of bacterial endophytes inhabiting Mimosa pudica L.
Research on endophytes is burgeoning immense importance since recent years with almost all plants harboring untold number of microorganism as endophytes. Endophytic plethora is reported to secrete unique novel metabolite...
Evaluation of Erythromycin–resistant and Chloramphenicol-sensitivity on Agrobacterium vitis in Iran
The purpose of this study was to determine the prevalence of Erythromycin and Chloramphenicol resistant/sensitivity of Agrobacterium vitis that isolated from environmental samples in Iran vineyards. From May to June 2014...
Prevalence of brucella abortus antibodies in bovine serum from gusau modern abattoir, Zamfara state, Nigeria
A study for bovine brucellosis was conducted using serology to determine the status of the disease in slaughtered cattle. Three hundred and twenty (320) sera were collected from Gusau Modern Abattoir, Zamfara State. The...