Abstract

Aim Many clinical and experimental studies have been made to prevent ischemia/ reperfusion (I/R) injury known as an important cause of organ dysfunction in lung transplantation (LT). In our study, we aimed to make a LT model in order to research I/R injury. Material and Methods In present study, we used 21 Sprague Dawney male rats weighting 200-225 gr. Three groups, each one including 7 rats, were formed. We named the fi rst group as control, the second one as donor and the third one as ischemia/ reperfusion group (IRG), respectively. We obtained sampling from the left lung lower lobe without any additional procedure in the control group for histopathological and biochemical evaluation. Left upper lobectomy and allograft left lower lobectomy were performed in the donor group (EG). During the removal of the left lower lobe, pulmonary artery and vein were catheterized to periphery. Left lower lobectomy was performed in IRG, as following the upper left lobectomy in the DG. Thus, left pneumonectomy was completed. Unlike the DG, the left lower lobe pulmonary artery and vein were catheterized to central. Aft er catheterization of the pulmonary artery and vein, allograft left lower lobe was reventilated and reperfused for two hours. The sampling from allograft lung was obtained for histopathological and biochemical study. The fi ndings and the results are statistically evaluated. Results Histopathologically, there were statistically signifi cant diff erences between CG and IRG in congestion, hemorrhage, parenchymal macrophages and PMN leukocyte infi ltration, emphysema, atelectasis, vacuolar degen¬eration and peribronchial lymphocytic infi ltration. We found increased mean MDA levels and GPx ac-tivities, decreased mean SOD and CAT activities. Conclusion IIn our study, we developed a LT model to research I/R, which does not require microsurgical procedure, has no complexity, inexpensive, modifiable and can be applied short time period.

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