ED50 from anti-inflammatory properties of Vitex trifolia L. ethanol extract
Journal Title: National Journal of Physiology, Pharmacy and Pharmacology - Year 2018, Vol 8, Issue 5
Abstract
Background: In Indonesia, Vitex trifolia grown as a crop or bush is even used as a guardrail. Conventionally, V. trifolia is used as a swelling drug caused by breast cancer by drinking the decoction of its leaves. Our group of researchers in previous articles has been able to prove scientifically that V. trifolia has anti-inflammatory activity using the carrageenan-induced method in five groups of rats. Because in the previous study found a large dose of 21.44%, 30.78%, and 41.46% for the dose of extract 500 mg / KgBW, 1000 mg / kg, and 2000 mg / KgBW for efficacious as anti-inflammatory, it is necessary to study the magnitude of the effective dose of the extract. Objective: This study aims to determine the median effective dosage of anti-inflammatory of V. trifolia L. ethanol extract. Materials and Methods: The dried V. trifolia leaves were finely chopped and then macerated with 96% ethanol in the macerator for 3 × 24 h, thickened with a rotary evaporator, and then evaporated over the water bath. Phytochemical screening was performed on the Farnsworth method. Against the extract, examination of extract parameters and TLC examination were performed based on Indonesian pharmacopoeia IV. Tests on ED50 were based on the induction procedure for inflammation of rats using carrageenan. The extract was administered orally to five test groups containing five mice at doses 1000, 1500, 2000, 2500, and 3000 mg/kg BW. The rat foot volume was measured and calculated percent inflammation inhibition after it was made a curve on probit log paper to determine ED50 extract. Results: From the result of substitution on plot equation of probit, log curve obtained effective dose median of Legundi leaf extract of 1428,8 mg/kg. Dose 1428.8 mg/kg BW was then defined as the ED50 anti-inflammatory activity of Legundi leaves extract. Through the calculation of one-way ANOVA and least significance different advanced test (P < 0.05), it could be proved that all the doses of the extract gave a significant difference to the negative control. The antiinflammatory activity was shown by the dose of extract 2000 m/kg BW, 2500 mg/kg BW, and 3000 mg/kg BW statistically did not differ significantly with positive control of 10 mg/kg BW. Conclusion: The effective dose of median (ED50) extract ethanol leaves V. trifolia Linn. which was efficacious anti-inflammatory was found 1428.8 mg/kg body weight. So far, no other researcher reported the ED50 of V. trifolia. These results suggest if the leaves legundi will be made anti-inflammatory herbal medicine, the preparation should be a drug to drink not a tablet considering the required dose big enough.
Authors and Affiliations
Resmi Mustarichie, Sri Adi Sumiwi, Annisa Trisfalia Hanifah
A comparative study of effect of cold pressor test on heart rate between healthy offspring with and without parental history of Type 2 diabetes mellitus
Background: Diabetes mellitus (DM) is a chronic metabolic disease characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. There is an increased susceptibility to develop the d...
Lipid profile and its relationship with blood glucose levels in Metabolic Syndrome
Background: Metabolic syndrome (MetS) and its associated factors such as dyslipidemia and hyperglycemia are associated with increased risk of cardiovascular disease (CVD). Aims and Objective: To assess lipid prof...
Relationship between short-distance run and variables of pulmonary function tests
Background: The relationship of pulmonary functions and running performance has always been a topic of debate among the fraternity of physiologists. The relationship of submaximal exercise with lactate threshold,...
Escitalopram-induced hyponatremia
Escitalopram is an antidepressant belonging to the class of selective serotonin reuptake inhibitors. It is effective in the treatment of generalized anxiety and major depressive disorders. It also has an off-labeled use...
A comparative study of lipid profile, body mass index, and waist circumference among Type 2 diabetes mellitus patients with poor and good metabolic control and normal age-matched control group
Background: Prevalence of dyslipidemia is very common in Type 2 diabetes mellitus (DM), and lipid abnormalities are likely to play an important role in the development of the atherosclerotic vascular disease. Type 2 diab...