EFFECT OF APIUM GRAVEOLENS EXTRACTS ON CASPASE-3, -8, -9 AND APAF-1 IN LNCaP CELLS
Journal Title: Kocatepe Medical Journal - Year 2024, Vol 25, Issue 3
Abstract
OBJECTIVE: In this study it was aimed to investigate the anti-cancer effect of ethanolic extracts of apium graveolens (celery) plant on cell viability, caspases involved in apoptosis and apoptosis protease activation factor-1 (Apaf-1) as inducer/suppressor in prostate cancer cell line LNCaP cells. MATERIAL AND METHODS: LNCaP cell line was used in our study. LNCaP cells were divided into 4 groups treated with ethanolic extracts of apium graveolens at increasing concentrations ranging from 0, 1500, 2000, 2500 μg/mL. The indicated extract concentrations were applied to LNCaP cells separately for 24 and 48 hours. Caspase-3, -8, -9 and Apaf-1 were measured by ELISA method from the cell lysate prepared at the end of the treatment. The results were expressed as ng/ml in proportion to the number of viable cells. RESULTS: Treatment of LNCaP prostate cancer cells with apium graveolens extracts for 24 and 48 hours did not cause a significant difference in caspase-3 and caspase-9 levels. However, caspase-8 levels were significantly increased at 2000 and 2500 μg/ml extract concentrations and Apaf-1 levels were significantly increased at 2500 μg/ml extract concentration in a dose- and time-dependent manner compared to the control groups at 24 and 48 hours. CONCLUSIONS: The results of this study show that ethanolic extracts of apium graveolens plant have anticancer and apoptotic properties by increasing caspase-8 and Apaf-1 levels in LNCaP prostate cancer cell line. Further in-vitro and in-vivo studies are needed to support our findings.
Authors and Affiliations
Halit Buğra Koca Tülay Köken Tülay Akan
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- EP ID EP740658
- DOI https://doi.org/10.18229/kocatepetip.1340255
- Views 48
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