Effect of different growth regulators on in vitro micro-propagation of Kufri Frysona
Journal Title: Journal of Applied and Natural Science - Year 2016, Vol 8, Issue 2
Abstract
In the present investigation, experiment was conducted for in vitro micro-propagation with different concentration of growth regulators in different explants Sprouts and Shoot tips of potato cultivar Kufri Frysona. The maximum survival percentage (40) of sprouts and (100%) of shoot tips were obtained when the explants were surface sterilized with 0.2% bavistin & 0.4% streptocyclin (45minutes) and 0.1% mercuric chloride (60seconds). Sterilized explants were inoculated on MS basal supplemented with various growth regulators and established successfully. The maximum shoot induction (62.5±1.44%) in 11.3±0.33 days and (74.0 ± 2.13 %) in 10.0 ± 0.50 days were reported on medium PM1 (BAP 0.25 mg/l) in sprouts and shoot tip explants respectively. The sprouted explants were further sub-cultured on MS media supplemented with various growth regulator alone and in combination for in vitro multiplication. In Kufri Frysona (11.2) shoots were obtained on MS medium fortified with 0.25mg/l BAP + 0.01mg/l IAA on 42th day of subculture. In vitro rooting was observed on MS basal medium supplemented with 2.0 mg/l NAA in Kufri Frysona after 10 days. Rooted plantlets were successfully hardened in green house using different types of potting mixture and finally transferred to field. The protocol will be very useful for large-scale production of disease free planting material of potato (S. tuberosum) in future.
Authors and Affiliations
Priyadarshani P. Mohpatra, V. K. Batra, Subhash Kajla, Anil K. Poonia, N. Manoj Kumar
Keywords
Related Articles
- EP ID EP284143
- DOI -
- Views 82
- Downloads 0
Composition, structure and diversity of tree species along an elevational gradient in Dudu forest range, Jammu and Kashmir, India
The present study has been undertaken along an altitudinal gradient (950-3500m a.s.l.) in Dudu Forest Range of Udhampur Forest Division, Jammu & Kashmir, India to understand the composition, structure and diversity of tr...
Induction of reactive oxygen species in brain of Etroplus maculatus after exposure to bisphenol A
The present study was aimed to investigate that bisphenol A, an environmental estrogen, exposed at 648 μg/ L concentration for 96 h could induce reactive oxygen species in brain of cichlid fish, Etroplus maculatus. Evalu...
Assessment of Penicillium bilaii inoculation in wheat (Triticum aestivum L.) for improving growth, yield and phosphorus availability in Mollisols of India
A field experiment was carried out with an aim to study the influence of two strains of Penicillium bilaii (PB -201 and PB-208) inoculation along with superphosphate application on growth, yield and P uptake of wheat (cv...
Allelopathic effect of Leucaena leucocephala on Pansy (Viola tricolor L.)
The present study on allelopahtic effect of L. leucocephala on pansy (V. tricolor L.) both laboratory and nursery conditions were undertaken. Leucaena which significantly reduced the seed germination in all treatments at...
Heterosis analysis in relation to drought tolerance in rice land races and their genotypes
An experiment was conducted in rice to study the heterosis for drought tolerance and grain yield in 24 hybrids developed from six landraces viz., Kallurundaikar, Kuliadichan, Kuruvaikalangiam, Mattaikar, Nootripathu and...