Effect of gamma rays on nuclear DNA content in different rose genotypes
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2012, Vol 3, Issue 6
Abstract
The effect of gamma rays on nuclear DNA content as an index of radiation damage in different genotypes of Rosa hybrida was investigated and the morphological characteristics of the resulting mutants were compared with their own progenitors. The in vitro nodal sections of five rose cultivars ("Apollo", "Maroussia", "Dolce vita", "Black baccara" and "Beauty by ogre") were irradiated by 0, 30, 60 and 90 Gy gamma rays. Nuclear DNA content of the putative mutants and their original plants was estimated by flow cytometry. The results revealed that nuclear DNA content was influenced by different doses of gamma rays. With increasing the dose, nuclear DNA content was decreased significantly. The decrease in nuclear DNA content was directly related to the plant genome size. Reduction in DNA content was greater in genotypes with larger genomes sizes than those with smaller genome sizes. The highest decrease in nuclear DNA content (0.33 pg) was related to the genotype "Black baccara", possessing the biggest genome size (2.49 pg) when irradiated with 90 Gy. The morphological characteristics of the putative mutants (treated with 60 Gy) were noted. The mutants that showed variation in their morphological characteristics did not have a significant difference in nuclear DNA content compared to the control plants. This suggests that changes in nuclear DNA content may not necessarily induce variation in morphological characteristics; moreover mutation may induce changes at the allele level rather than the alterations in the nuclear DNA content which is detectable by flow cytometry.
Authors and Affiliations
Zahra Aghcheh Kahrizi| Department of Tissue culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran.Department of Horticultural Science, Faculty of Agriculture, Zanjan University, Zanjan, Iran, Maryam Jafarkhani Kermani*| Department of Tissue culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran.m.j.kermani@abrii.ac.ir, Mohammad Amiri| Department of Horticultural Science, Faculty of Agriculture, Zanjan University, Zanjan, Iran
Keywords
Related Articles
- EP ID EP5024
- DOI -
- Views 453
- Downloads 24
Vernacular Techniques of Using Wind in order to Rehabilitate the Identity of Contemporary Rural Housing Architecture of Sistan
The main concern of this article is the unplanned and uncontrolled construction of Sistan (located in eastern Iran) rural housing developers. They have designed contemporary rural housing of this region without consideri...
Effect of Methyl Jasmonateon Chloride Andnitrate Concentrations, Nitrate Reductaseactivity, Malondialdehyde Content And Peroxidase Activity in Nicotianatabacum l. Cv. Coker 347 in Different Concentrations Of Chloride
Jasmonate (Jasmonic acid and its methylated ester, methyl jasmonate) are a new group of plant growth regulators. These compounds reduce damage from dehydration, cold and salinity. In this study, methyl jasmonate (30?m) w...
Study the causal relationship between brand personality to Brand trust and brand affect in consumer sport
The aim of the present study was to investigate causal relationship with the brand personality to Brand trust and brand affect in consumer sport. In this research, causal and data collection tools is a scale developed by...
Geology,Petrography and geochemical of the Selijerd Intrusive Rocks, Northeast Saveh, Central Iran
The Selijerd intrusive mass is located in the Northeast of Saveh and is considered as a part of the Urumieh-Dokhtar magmatic arc (UDMC). The mass is replaced in the Eocene-Oligocene volcanosedimentary rocks consist mainl...
Interaction between Sclerotinia sclerotiorum virulence and important crop's resistance
In order to study on pathogen virulence and plant resistant interaction, out of more than 400, 30 isolates obtained from different hosts including lettuce, sunflower, cabbage, rape-seed, tobacco, soybean and artemisia we...