Effect of Purification, Chemical Factor and Shear Stress on Endothelial Differentiation of Human Adipose-Derived Mesenchymal Stem Cells using a Perfusion Bioreactor
Journal Title: International Journal of Stem Cell Research and Transplantation (IJST) - Year 2016, Vol 4, Issue 7
Abstract
The objective of this study was to answer to these principal questions that whether a more homogeneous and purified colony of Human adipose-derived mesenchymal stem cells (hASCs) has significantly higher ability of differentiation in comparison to heterogeneous hASCs colony? And also is the any synergism between chemical and mechanical motivation of hASCs? For this purpose, CD-271+ hASCs were isolated by flow cytometry approach and their differentiation ability toward endothelial cells (ECs) was analyzed after application of mechanical shear stress and chemical growth factor. The results were compared to that of heterogeneous colony of hASCs. Abdominal adipose tissues were isolated from previously informed and consent 25-35 year old healthy women. A perfusion bioreactor was used for application of flow shear stress of magnitude of 4.64 dyne/cm2 purified and nonpurified hASCs which lie on collagen type I coated silicon scaffold. In addition to that, cells were exposed to 50 ng/ml vascular endothelial growth factor (VEGF) for 7 days. Three endothelial specific genes (FLK-1, vWF and VE-cadherin) were selected and their expressions in RNA level were assessed by real time PCR. Real time PCR results demonstrated that generally CD-271+ hASCs have more promising differentiation ability. Also it has been shown that highest expression of ECs specific genes is related to concurrent chemically and mechanically motivated hASCs. In conclusion, mechanical stimulation is at least as important as chemical stimulations and a more homogeneous group of hASCs could be regarded as a more convenient source of cells for vascular tissue engineering applications.
Authors and Affiliations
Ghasem Amoabediny, Nooshin Haghighipour,
Keywords
Related Articles
- EP ID EP198066
- DOI 10.19070/2328-3548-1600034
- Views 99
- Downloads 0
Effect of Snake Venom Disintegrin like domain on the Homing of Mesenchymal Stem Cells
Mesenchymal stem cells have many advantages as grafts for cell transplantation. The homing of MSCs after systemic infusion is still poorly understood. This report explored the effect of the combination of BM-MSCs with Di...
Human Umbilical Cord Matrix - Derived Stem Cells Show Tropism Towards the Human Glioma Microenvironment
Objective: Glioblastoma multiforme (GBM) is an aggressive type of brain tumor in humans. The median survival of patients is a year after diagnosis. It is a great challenge to identify new therapeutic strategies that coul...
Indirect Co-Culture with Schwann Cells as a New Approach for Human Endometrial Stem Cells Neural Transdifferentiation
Neurodegenerative diseases such as Spinal Cord Injury (SCI) refer to the loss of neuronal cells in the central nervous system. Cell replacement therapy is currently the most propitious approach for the treatment of such...
Epigenetic Regulation of Retinal Stem Cells
Epigenetic Regulation of Retinal Stem Cells
Central Pontine Myelinolysis Associated with Cyclosporine in a Patient with NK Cell Leukaemia after Hematopoietic Stem Cell Transplantation
Central pontine myelinolysis (CPM) has been described as a rare complication of liver transplantation. Cases in allogeneic hematopoietic stem cell transplant are less reported. We present a patient with NK cell leukaemia...