EFFECT OF PYRACLOSTROBIN ON INDUCTION OF PATHOGENESIS RELATED PROTEINS IN BRINJAL (SOLANUM MELONGENA L) AGAINST PHOMOPSIS VEXANS
Journal Title: International Journal of Agriculture Sciences - Year 2016, Vol 8, Issue 8
Abstract
In the present study, the effect of Pyraclostrobin treatment on induction of pathogenesis related proteins in a susceptible brinjal cultivar (cv. PPL) was studied upon challenge inoculation with Phomopsis vexans_MK2 isolate. Seeds treated with sterile distilled water served as controls. Seeds treated with 0.2% Pyraclostrobin along with respective control seeds (cv. PPL) were raised under nursery bed. Thirty days old seedlings were challenge inoculated with conidial suspension of P. vexans (1 x106 conidia/ml). Seedlings were harvested at regular intervals of 0, 3, 6, 12, 24, 48, 72 & 96 hours of post inoculation and analyzed for activity of pathogenesis related proteins (PRP’s) namely β -1,3-glucanase, chitinase and lipoxygenase, colorimetrically. The results revealed that Pyraclostrobin treatment enhanced the activity of pathogenesis related proteins when compared to control seedlings (water inoculated, challenge inoculated with P. vexans) Significant differences (P - 0.001) in activity of β -1,3-glucanase was observed at 48hpi upon Pyraclostrobin treatment followed by challenge inoculation with P. vexans. An increased chitinase(P-0.03) activity was also recorded at 48-96 hpi upon Pyraclostrobin treatment and lipoxygenase activity was found maximum at 48 hpi. The results indicated thatPyraclostrobinhave a positive effect on the induction and activity of defense related proteins like β -1,3-glucanase, chitinase and lipoxygenase.
Authors and Affiliations
S. MAHADEVAKUMAR
Keywords
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