Effect of voltage-gated sodium channels blockers on motility and viability of human sperm in vitro
Journal Title: Asian Pacific Journal of Reproduction - Year 2018, Vol 7, Issue 2
Abstract
Objective: To test the effect of voltage-gated sodium channels (VGSCs) blockers on the motility and viability of human sperm in-vitro and to evaluate the tested compounds as potential contact spermicidal. Methods: Sperm samples were obtained from healthy non- smoking volunteers of age 25-30 years who had not taken any drug 3 months before and during the course of the study. The effect of VGSCs blockers evaluated from two pharmacological classes including antiarrhythmic (amiodarone, procainamide and disopyramide) and antiepileptic (carbamazepine, oxcarbazepine, phenytoin, and lamotrigine) drugs. They were tested on the in-vitro motility and viability of human sperm using Computer Assisted Semen Analyzer. Results: All tested drugs except oxcarbazepine showed dose dependent inhibition of total motility with significant reduction (P<0.05) at the maximum concentration of 200 µM when compared with the control. The concentrations of drugs that reduced total sperm motility to 50% of control (half maximal inhibitory concentration) were 2.76, 14.16 and 20.29 µM for phenytoin, lamotrigine and carbamazepine, respectively; and 2.53, 5.32 and 0.37 µM for amiodarone, procainamide and disopyramide, respectively. The anti-motility effects were reversible to various degrees. There was statistically insignificant difference in the inhibition of sperm viability among amiodarone, procainamide and disopyramide. Phenytoin demonstrated the most potent spermicidal action. Conclusions: VGSCs blockers have significant adverse effects on in-vitro motility of human spermatozoa. So in-vivo studies are required to determine their potential toxicological effects on human semen quality, which is an important factor regarding fertility. Moreover, these drugs have the potential to be developed into contact spermicidal.
Authors and Affiliations
Hammad Ahmad Gakhar, Ishrat Waheed, Taseer Ahmad, Naeem-ur -rahman
Keywords
Related Articles
- EP ID EP407442
- DOI 10.4103/2305-0500.228015
- Views 205
- Downloads 0
Biofertilizing efficiency of Sargassum polycystum extract on growth and biochemical composition of Vigna radiata and Vigna mungo
Objective: To evaluate the effect of marine brown alga Sargassum polycystum extract on growth and biochemical parameters of Vigna radiata and Vigna mungo. Methods: Different concentrations of algal extracts (0.5%, 1.0%,...
Secondary sex ratio of assisted reproductive technology babies
Objective: To assess the secondary sex ratio (SSR) of assisted reproduction technology babies within a fertility clinic in Melbourne, Australia. Methods: A retrospective cohort study was conducted on 3 369 babies who wer...
Orexins and male reproduction
Orexins (or hypocretins) are hypothalamic neuropeptides with a multitude of physiological functions. They occur in two known forms, namely, orexin A and orexin B with a common precursor, preproorexin. The orexin receptor...
Comparison of transvaginal cervical length and modified Bishop’s score as predictors for labor induction in nulliparous women
Objective: To compare the transvaginal cervical length (TVCL) to the modified Bishop’s score for prediction of successful labor induction in nulliparous women. Methods: A total of 210 nulliparous women who were diagnos...
Effect of using ascorbic acid and cysteamine supplementation on in-vitro development of buffalo embryos
Objective: To improve in vitro embryo production in buffalo by supplementation of L-ascorbic acid during maturation and development (experiment 1) and combination with another antioxidant as cysteamine (experiment 2). Me...