Effective Aeromonas specific monoclonal antibody for immunodiagnosis
Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2018, Vol 8, Issue 1
Abstract
Objective: To identify the monoclonal antibody specific to Aeromonas spp., a Gram negative bacteria causing gastroenteritis and wound infection. Methods: The monoclone, namely 88F2- 3F4, was produced from hybridoma technology. The specificity of antibody secreted from 88F2-3F4 was tested against other Gram negative bacteria frequently found in gastrointestinal tract. Then the antibody was used for searching Aeromonas antigens in artificial seeded rectal swab cultures by dot-blot enzyme linked immunosorbent assay. Results: 88F2-3F4 produced an antibody that recognized an antigen with a molecular mass of 8.5 kDa in all 123 isolates of the seven Aeromonas species tested, but recognized no epitope of any other Gram-negative bacterium typically found in the gastrointestinal tract. A dot-blot enzyme linked immunosorbent assay based on this antibody showed 86.49% sensitivity and 92.13% specificity. Conclusions: 88F2-3F4 monoclonal antibody could react with all Aeromonas isolates, but not other Gram negative bacteria, therefore it should be a useful tool for the detection of Aeromonas antigen in clinical and environmental samples.
Authors and Affiliations
Yuvadee Mahakunkijcharoen, Chakrit Hirunpetcharat, Sunisa Malijunbua, Watcharamat Muangkaew, Suporn Paksanont
Keywords
Related Articles
- EP ID EP370207
- DOI 10.4103/2221-1691.221128
- Views 74
- Downloads 0
Potential therapeutic effects of thymoquinone on treatment of amphetamine abuse
None
Infections and inequalities: anemia in AIDS, the disadvantages of poverty
Objective To study anemia in AIDS patients and its relation with socioeconomic, employment status and educational levels. Methods A total number of 442 patients who visited the Infectious Diseases University Hospital...
Anti-angiogenic effect of Artocarpus heterophyllus seed methanolic extract in ex ovo chicken chorioallantoic membrane
Objective: To examine the anti-angiogenic potential of Artocarpus heterophyllus (A. heterophyllus) seed extract in chicken chorioallantoic membrane (CAM). Methods: This study used chicken CAM ex ovo culture to examine th...
Anti-hemolytic, antibacterial and anti-cancer activities of methanolic extracts from leaves and stems of Polygonum odoratum
Objective: To investigate anti-hemolytic, antibacterial and anti-cancer activities of leaf and stem extracts from Polygonum odoratum. Methods: Leaves and stems of Polygonum odoratum were extracted using methanol and thei...
Molecular detection of Anaplasma marginale and Anaplasma ovis in sheep and goat in west highland pasture of Iran
Objective: To determine the prevalence of Anaplasma marginale (A. marginale) and Anaplasma ovis from sheep and goat in different highland pasture in west of Iran. Methods: From July 2015 to October 2015, 370 blood sample...