Effects of administration of co-trimoxazole and folic acid on sperm quality and histological changes of testes in male rats
Journal Title: International Journal of Reproductive BioMedicine - Year 2017, Vol 15, Issue 10
Abstract
Background: Male infertility has been reported following long-term sulfasalazine, however, the precise effects of co-trimoxazole on sperm quality is controversial. Objective: In this study, we evaluated the effects of co-trimoxazole and its coadministration with folic acid on sperm quality and histological changes of testes in male rats. Materials and Methods: In this experimental study, 136 male Wistar rats were divided into 9 groups: I (control), II (vehicle) received saline, III: received folic acid (1 mg/kg /daily i.p., and IV- IX received co-trimoxazole (30, 60, and 120 mg/kg/daily; i.p.)+folic acid (1 mg/kg/daily; i.p.) for 14 or 28 days. Sperm samples were obtained from each group at the end of 14th and 28th days. Sperm numbers, motility, and viability were evaluated on a hemocytometer. Hematoxylin and Eosin stained testes were done for evaluation ofthe number of Leydig cells, vascularity, spermatids, spermatocytes, and means of seminiferous tubules diameter under light microscopy. Results: Co-trimoxazole treatment for either 14 or 28 days caused a significant decrease in the percentage of sperm number, motility, and viability (p<0.001) compared to the control group. Also, high doses of co-trimoxazole caused a significant decrease in testes structural abnormalities means of seminiferous tubules diameter, spermatids, and spermatogonia) compared to the vehicle group (p<0.001). Folic acid co-administration with co-trimoxazole partially reversed the decrease in sperm quality and structural abnormalities of high doses of co-trimoxazole (60 and 120 mg/kg/daily) (p<0.001). Conclusion: The data showed the adverse effects of co-trimoxazole on sperm quality and testes morphology which was protected partially by folic acid coadministration in rats. The underlying mechanism (s) needs further investigations.
Authors and Affiliations
Ehsan Salarkia, Gholamreza Sepehri, Parvin Torabzadeh, Jalil Abshenas, Arezoo Saberi
Keywords
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- EP ID EP281282
- DOI 10.29252/ijrm.15.10.5
- Views 110
- Downloads 0
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