Effects of Ethanolic Extract of Bauhinia Variegata Leaves Alone and In Combination with Metoclopramide on Induced Model of Acute Colitis in Rabbits
Journal Title: Scholars Academic Journal of Pharmacy - Year 2018, Vol 7, Issue 2
Abstract
Abstract: The exact reasons of UC are still unidentified; however, numerous factors have been implicated in the pathophysiology of the disease. Inflammatory mediators such as cytokines, reactive oxygen species, also 5-HT plays a key task in the pathogenesis of UC. The available drugs for the treatment of UC have considerable systemic side effects. Therefore, there is a pressing need for the development of new therapeutic agents for the treatment of UC, Thus, the evolution of a new safe, effecient and cheap medicine is required. The aim of current study is to investigate the effect of ethanolic extract of Bauhinia variegates alone or in combination with metoclopramide on inflammatory biomarkers, oxidative stress parameters and histopathological score in experimentally induced colitis. This study was conducted on thirty six male albino weighting about (1000-1750) g rabbits were brought from animal house previously submitted to starvation for at least 12hrs .Animals were divided into six groups (n=6/group). All groups except group I (served as normal control and received no treatment) were received 2ml acetic acid rectally .Group II served as a colitis control and were received only acetic acid rectally. Group III , IV , V and VI rabbits with colitis treated rectally with mesalazine 100mg/kg, etanolic extract of Bauhinia variegata 200mg/kg and metoclopramide 0.5mg/kg and combination of Bauhinia variegate plus metoclopramide respectively for 5days. All these drugs were given for five days rectally. After the end of experiment , blood collected from animals for measurement of the serum cytokines (Tumor necrosis factor-alpha and Interlukin-6) by ELISA kits, then sacrificed by an over dose of diethyl ether inhalation and then the abdomen was rapidly dissected and open the colon was removed and submitted to microscopical assessment(histopathological score) and the measurement of the colonic tissue of lipid peroxide and glutathione peroxidase by ELISA kits.Combination produced the significant reduction in histological score in comparison with ethanolic extract of Bauhinia variegata , metoclopromide, (P<0.01), 1 ±0 , 1.11 ±0.06, 2 ±0, respectively, but less than mesalazine which is0.9±0.04.Combination produced the significant reduction in colonic tissue of lipid peroxide in comparison with ethanolic extract of Bauhinia variegata, metoclopromide (P<0.01) and its effect was comparable to that produced by mesalazine (P>0.05) 10.04 ± 1.21, 10.67 ± 1.09 , 13.87±1.31, 9.5 ± 1.88 respectively. Combination produced the significant reduction in colonic tissue of glutathione peroxidase in comparison with ethanolic extract of Bauhinia variegata, metoclopromide (P<0.01) and its effect was comparable to that produced by mesalazine (P>0.05) 37.11 ± 1.12, 36.04 ± 1.83, 27.26±1.98, 37.56 ± 1.63 respectively. Combination produced the significant reduction in serum level of tumor necrosis factor alpha in comparison with ethanolic extract of Bauhinia variegata, metoclopramide (P<0.01) and its effect was comparable to that produced by mesalazine (P>0.05) 16.86± 0.6, 18.34 ± 1.26, 29.09 ± 1.28, 14.76 ±0.77 respectively. Combination produced the significant reduction in serum level of interlukin-6 in comparison with ethanolic extract of Bauhinia variegata , metoclopromide (P<0.01) and its effect was comparable to that produced by mesalazine (P>0.05) 89.97 ± 1.08 ,91.01±1.58, 119.05 ± 1.03 , 87.09 ± 1.88 respectively.
Authors and Affiliations
Mustafa Hussein Sachit, Ahmed Rahma Abu Raghif, Shihab Abdul Rhman Shihab
Keywords
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