Effects of frozen diluents on storage of ram sperm
Journal Title: International journal of Advanced Biological and Biomedical Research - Year 2013, Vol 1, Issue 12
Abstract
Semen collection evaluation and addition of preservatives to increase storage period of sperm are essential for successful artificial insemination. This study was conducted on 4rams (2 Ghezel Merinos and 2 Merinos Moghani) to evaluate the effect of two diluents in Khalatposhan research station. Average age of rams was 3 years. Rams were trained to serve the artificial insemination and semen samples were collected weekly. It was started from October 2011 to June 2012. After collection semen samples were mixed with diluents. They were stored in liquid nitrogen. After diluting and storage semen was assessed after 0, 1, 2 and 3 day for pH, viability and progressive motility. Frozen straws were thawed individually at 37 °C for 20 s in a water bath for evaluation. Effect of diluent and storage day on pH, viability and progressive motility of sperm were significant (P< 0.01). With the increasing storage day pH, viability and progressive motility of sperm decreased. The success of these diluent has been attributed the tris which may act as a buffer against changes in pH and tonicity. Fructose and citric acid are energy source. Egg- yolks protect the cell membrane during cooling. Glycerol protects the spermatozoa against membrane damage during freezing. This study showed that diluents containing of 7% glycerol and 20% egg-yolk had better sperm protection ability than extender containing 5% glycerol and 5% egg-yolk according to sperm motility, pH and sperm viability.
Authors and Affiliations
Fatemeh Soltanpour| M.Sc. Student of Animal Physiology, Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran, Gholamali Moghaddam| Professors, Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran, E-mail: ghmoghaddam@tabrizu.ac.ir
Keywords
Related Articles
- EP ID EP12967
- DOI -
- Views 357
- Downloads 13
Fracture Mechanics Analysis of Fourth Lumbar Vertebra in Method of Finite Element Analysis
Objective: In this paper, finite element model of the L4 vertebra subjected to combination of compression and flexion loading in isotropic and anisotropic cases is investigated. Methods: In both cases, the vertebra is...
Effective of Sick Building Syndrome on Irritation of the Eyes and Asthma
In this research, we investigated basic symptoms of syndrome in illnesses of sick buildings. We designed a questionnaire and asked from male of dormitory residence. Questions of the questionnaire were more about known...
Functional assessment of CODM gene in different cultivar of Papaveraceous species via in silico analysis
Medicinal use of the opium poppy (Papaver somniferum L) has ancient history, but the isolation of morphine was not described until the early nineteenth century. Morphine is the most important alkaloid of opium poppy in...
SWI: Probe for neuroradiologists
Susceptibility-weighted imaging (SWI) has continued to develop into a powerful clinical tool to visualize venous structures and iron in the brain and to study diverse pathologic conditions. It is a new art which evaluate...
Evaluation of genotype × environment interaction in chickpea genotypes using path analysis
Chickpea (Cicer arietinum L.) is one of the most important crops worldwide that is a subject of many plant breeding programs in many countries. In addition, climates are changing all over the world and drought becomes...