Effects of oxidized glutathione, cysteine and taurine supplementations on motility charateristics of different goat spermatozoa types
Journal Title: Iranian Journal of Veterinary Science and Technology (IJVST) - Year 2021, Vol 13, Issue 2
Abstract
This study aimed to determine the influences of antioxidants additives on motility characteristics of different goat spermatozoa types including frozen/thawed, fresh, and chilled samples. Ejaculates from five Shami bucks were collected during breeding and non-breeding seasons. Spermatozoa samples from the three types were incubated in media containing 2 and 4 mM oxidized glutathione (GSSG), 5 and 10 mM L-cysteine,10 and 25 mM taurine, and no additives (control). Motility characteristics were analyzed by a computer-aided sperm analyzer (CASA). Except for taurine, the addition of antioxidants resulted in a significant (p < 0.05) increase in the percentage of motile sperm (MOT %) after spermatozoa thawing. When fresh sperm samples were collected during the non-breeding season and treated with both GSSG and L-cysteine, the values of the velocity parameters VAP, VSL, and VCL increased significantly (p < 0.05). No significant effects were noted for the velocity parameters when 10 and 25 mM of taurine were added to the chilled spermatozoa, while GSSG and L-cysteine had principally affected MOT % of this spermatozoa type. The rapid spermatozoa subpopulation was the most influenced category by the three antioxidants compared to the slow and medium grades, especially in the case of fresh and frozen/thawed types. In conclusion, the effects of different antioxidants on goat spermatozoa motility largely depend on the used concentration and also on the type of spermatozoa pattern.
Authors and Affiliations
Mazen Alomar,
Keywords
Related Articles
- EP ID EP722361
- DOI -
- Views 59
- Downloads 1
Protective Effect of Celery ( Apium graveolens L. ) Essential Oil on the Experimental Model of Cuprizone-induced Multiple Sclerosis in Male C57BL/6 Mice
Considering the beneficial effects of Apium graveolens L. (Celery) on the nervous system, this study elucidates the protective effect of CEO on the experimental model of cuprizone-induced MS in male C57BL/6 mice. Frothy...
A serological survey on Leptospiral infection in squirrels and hamsters in Ahvaz district, SouthWest of Iran
Leptospirosis is a zoonotic infectious disease of worldwide distribution. The infection is caused by various serovars of Leptospira interrogans sensu lato. Although the squirrels and hamsters are considered to be the res...
Chromosomal analysis of two buffalo breeds of Mazani and Azeri from Iran
In the present study karyotype of Mazani river buffalo was studied in comparison with those of Azeri buffalo populations from Iran. Blood samples were taken from ten (5 males and 5 females) Mazani buffaloes and thirty (1...
Assessment of some inflammatory cytokines and immunologic factors in dairy cows with subclinical ketosis
Altered cytokine profile and weakened immunity along with clinical or subclinical ketosis (SCK) are among the remarkable challenges around parturition. Therefore, the present study aimed to compare some inflammatory cyto...
Prevalence assessment of Salmonella serovars in apparently healthy pet dogs in Tehran, Iran
Salmonellosis is considered to be a zoonotic disease, the transmission of which through oral-fecal contact is unavoidable because pet care has been popular recently. On the other hand, excessive use of human antibiotics...