Effects of salinity on the DNA methylation pattern in barley shoots
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2017, Vol 10, Issue 1
Abstract
Salinity is a crucial factor which inhibits crop production worldwide. Recent pieces of evidence reveal that epigenetic mechanisms modulate the gene expression in plants undergoing environmental stresses. Obviously, when the epigenetic regulation of plant growth and response to these stresses are truly understood, a novel heritable variation could be developed for crop improvement. The present study attempted to evaluate the DNA methylation alteration made by salt stress in two barley (Hordeum vulgare L.) cultivars differing in salt tolerance, namely salt-tolerant Sahara3771 and salt-sensitive Clipper. Coupled Restriction Enzyme Digestion-Random Amplification (CRED-RA) was used to detect changes in the methylation pattern of the sequence CCGG in the nuclear genome of the plants growing under salinity stress (100 mM NaCl) and normal conditions. Leaf samples for DNA extraction were harvested 24 hours, 3 weeks, and 5 weeks after salt treatment. The results revealed that the average number of sites showing an increase in the methylation level at the three growth stages with the salt-stress imposition was higher in Sahara3771 (26.21%) than in Clipper (16.32%). Moreover, the number of sites with an increase in methylation under salt stress in Sahara3771 and Clipper, 24 hours and 5 weeks after imposing stress, respectively, was higher than the number of sites at the other stages. These results indicated a significant alteration of DNA methylation in plants as a response to salt stress and the effect was dose-dependent. These changes could provide a mechanism for the adaptation of plants under salt stress.
Authors and Affiliations
Samira Hamian, Seyyed Abolghasem Mohammadi, Mohammad Moghaddam, Ali Bande Hagh
Keywords
Related Articles
- EP ID EP39008
- DOI -
- Views 182
- Downloads 0
Obstacles of ranchers` participation in implementing the pastures management plans in Kohgeloyeh township of Iran
Pastures are one of the natural resources` important having a key role in each country's water and soil protection. They, in Iran, are considered as one of the most important resources. This study is aimed at identifyin...
Leather Dyeing with Plants Dyes: A Review
The paper reviewed the application of natural dyes on leather. The analysis of reviewed articles showed that different colours were obtained with the use of common and traditional mordants such as copper sulphate, ferrou...
Genetic diversity and kinship of ebony population (Diospyros celebica Bakh.) in its natural population in Central Sulawesi
Diospyros Celebica Bakh. is a type of Sulawesi endemic that has a high economic value, but its existance becomes decreased due to excessive exploitation and no proper replanting. Conservation and plant development is urg...
Describing body shape variation of bugwan fish (Hypseleotris agilis) species using land mark-based geometric morphometric in Lake Mainit, Surigao del Norte, Philippines
The study primarily aimed to determine the body shape variation of Hypseleotris agilis using Fluctuating Asymmetry (FA) in relation to their existing population in Lake Mainit, Surigao del Norte, Philippines. The researc...
Exploration of floristic diversity of Khanpur valley, District Haripur, Khyber Pakhtunkhwa, Pakistan
A floristic inventory was carried out during 2012-2014 in Khanpur Valley, District Haripur, Khyber Pakhtunkhwa, Pakistan. Data pertaining to floral composition and other relevant information was collected through questio...