Effects of Trehalose and Sucrose on Human Sperm Motility, Vitality and Morphology after Cryopreservation
Journal Title: Journal of Health Science and Medical Research (JHSMR) - Year 2019, Vol 37, Issue 2
Abstract
Objective: To compare human sperm motility, vitality and morphology after cryopreservation among sucrose, and different trehalose concentrations. Material and Methods: A total number of 124 normozoospermic semen samples were collected. Each semen sample was divided into 4 portions, and cryopreserved in a human sperm-preserving medium along with cryoprotectants, including; 50 milimolar (mM) trehalose, 100 mM trehalose, 200 mM trehalose and 50 mM sucrose, respectively. All semen samples were frozen by using a vapor phase method. Post-thawed sperm motility, vitality and morphology were assessed. R program was used for data analysis. A p-value of <0.05 was considered statistically significant. Results: Post-thawed semen evaluation indicated that 50 mM trehalose was better than 50 mM sucrose in all sperm parameters, which included progressive motility (p-value=0.037, total motility (p-value<0.001), vitality (p-value<0.001) and morphology (p-value<0.001). The sperm parameters were not significantly different among 100 mM trehalose, 200 mM trehalose and 50 mM sucrose. Conclusion: The use of 50 mM trehalose, as non-permeating cryoprotectant, showed superior post-thaw sperm parameters over sucrose, and other trehalose concentrations.
Authors and Affiliations
Manaphat Suksai, Kriengsak Dhanaworavibul
A Comparison Study of the Auditory P300 Results of Elderly Patients with Sensorineural Hearing Loss
Objective: The purpose of this study was to compare the auditory P300 results of elderly patients with sensorineural hearing loss (SNHL) before and after hearing aid use. Material and Methods: A cross-sectional study wa...
Improvement of the Osmotic Fragility (OF) Test in α Thalassemia 1 Screening for HIV-1 Infected Patients by The New Cutoff Values of MCV and MCH
Objective: To evaluate the efficiencies of the standard α thalassemia screening regimen in human immunodeficiency virus (HIV) infected patients and improve the efficiencies of this screening regimen using new cutoff valu...
Regulation and Decriminalisation of Illegal Substances in Thailand
Psychoactive substances – chemical compounds which can alter a person’s mood, thoughts, and behaviors may be liable to misuse and cause addiction. Internationally, many strategies have been implemented in order to limit...
Effectiveness of using the Centering Teen Pregnancy Program on Postpartum Depression among Adolescent Mothers: A Posttest Only Quasi-experimental Design using a Comparison Group
Objective: To examine the effectiveness of the Centering Teen Pregnancy Program on postpartum depression among adolescent mothers. Material and Methods: Study sample was 60 adolescent mothers who attended a prenatal car...
Evaluation of an Epidemiological Surveillance System for Preterm Birth and Low Birth Weight in Southern Thailand
Objective: To assess the prevalence and management of preterm birth and low birth weight (LBW) infants, including the exploration of known risk factors for preterm birth and LBW, recorded in field testing of a web-based...