Embryogenic potential and the expression of arabinogalactanoproteins (AGPS) in embryogenic cell aggregates of Coffea arabica cv. Caturra Rojo under the influence of CO2
Journal Title: Biotecnología Vegetal - Year 2002, Vol 2, Issue 3
Abstract
The establishment and optimization of regeneration systems via somatic embryogenesis has centered traditionally on the study of the culture medium components, with little attention to other factors of the in vitro environment like the composition of the gaseous atmosphere. This work was realized with the aim to determine the embryogenic potential and the expression of arabinogalactanproteins (AGPs) in embryogenic cell aggregates of Coffea arabica cv. Caturra Rojo under influence of carbon dioxide (CO2). Concentrations of 2.5% and 5.0% of CO2 stimulated a greater formation of somatic embryos (307 ES/50mgMF and 277 ES/50mgMF respectively) in semisolid culture medium with better results to the controls with passive interchange and forced ventilation (195 ES/50mgMF and 107 ES/50mgMF) whereas a concentration of 10.0% of CO2 inhibited the process of somatic embryogenesis (95 ES/50mgMF). In cell suspensions the formation of somatic embryos was greater with a concentration of 2.5% of CO2 (130x103 ES.l-1) in comparison with 5.0% and 10.0% of CO2 (116x103 ESl-1 and 15x103 ES.l-1) and the controls with passive interchange and forced ventilation. With a concentration of 2.5% CO2 there was a greater synthesis of Arabinogalactanproteins (AGPs) at the cellular level (0.068 mg.gMF-1) and the excretion in the culture medium (2.10 mg.l-1), which coincided with the highest values of production of somatic embryos.
Authors and Affiliations
Raúl Barbón, Walter Preil, Elio Jiménez
Keywords
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