Enhancing the sensitivity of HIV DNA detection: a magnetic bead-based method
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 12
Abstract
Objective To establish a magnetic bead enrichment strategy for the detection of human immunodeficiency virus deoxyribonucleic acid (HIV DNA) in peripheral blood, and to verify the improvement of the sensitivity of this method for the detection of HIV DNA in HIV infected patients after early antiretrovital treatment (ART). Methods Peripheral whole blood was collected at 4 timepoints in one ART HIV window period (WP) patient. Peripheral blood mononuclear cells (PBMCs) were isolated on a Ficoll gradient. CD4+ T lymphocytes were enriched from total PBMCs by negative sorting. HIV DNA concentration in magnetic beads enriched group and whole blood group was detected by HIV DNA detection kit. Results CD4+ T cells were isolated by magnetic beads and identified by FCM for purity at (96.4 ± 2.6)%. The viability was (95.9 ± 2.9)%, as demonstrated by trypan blue staining. The person on continued ART treatment in this study had significantly greater reduction in HIV viral load and undetectable HIV plasma RNA at follow up timepoint 4. No HIV DNA was detected in the whole blood group at all 4 timepoints. The quantitative results of HIV DNA in the CD4+ T lymphocyte group of the magnetic bead enrichment group were 73.4, 429.3, 137.1, 449.9 copies/106 CD4+ T cell′s respectively. Conclusion The magnetic bead enrichment method can be more sensitive in detecting the limit low copy HIV DNA in blood samples, and provide early confirmatory data for HIV WP infection and breakthrough infection after ART treatment.
Authors and Affiliations
Lilin WANG, Linfeng WU, Fang ZHAO, Ran LI, Tong LI, Heng LIU, Rui ZHU, Jinfeng ZENG
Keywords
Related Articles
- EP ID EP738715
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.12.006
- Views 46
- Downloads 0
Investigation and analysis of HbA1c among voluntary blood donors in Hunan Province
Objective To understand the abnormalities of HbA1c in the blood donor population in order to improve blood quality and blood donor safety. Methods The qualified voluntary blood donors, from April 2021 to March 2022, were...
Molecular biological analysis of RhD variant blood donors in Zhengzhou
Objective To investigate the serological characteristics and gene mutation mechanism of RhD variant blood donors in Zhengzhou. Methods From January 2023 to December 2023, 1 619 RhD-negative blood donors sent to our labor...
Syphilis screening results of blood donors in Yinchuan from 2018 to 2021
Objective To analyze the syphilis(TP) screening results of blood donors in Yinchuan from 2018 to 2021, and to provide scientific basis for assessing the risk of syphilis infection among the blood donors. Methods Retrospe...
Application and prospects of homologous allogeneic platelet-rich plasma in wound repair
The application of platelet-rich plasma (PRP) in wound repair is progressively garnering attention. However, certain patients, owing to relative or absolute contraindications, encounter impediments in the acquisition or...
Study on the production efficiency of platelet components in 24 prefecture-level blood stations in China
Objective To learn the production efficient of platelet components among prefecture-level blood stations in China, to provide supporting data for those blood stations to optimize the production mode of platelet component...