Epidermal Growth Factor Receptor Mutations detection by Mutant Specific Immunohistochemistry in North Indian Lung Cancer population

Abstract

Background: Lung cancer is one of the leading causes of cancer-related deaths due to limited treatment options available for advanced-stage disease. The epidermal growth factor receptor is a trans-membrane glycoprotein having an extracellular epidermal growth factor binding domain and an intracellular tyrosine kinase domain which regulates signaling pathways to control cellular proliferation. Material and Methods: Detection of EGFR mutation status of 80 subjects of non-small cell lung cancer of adenocarcinoma subtype had been done by IHC using EGFR mutation specific antibodies. Results: 20 cases were positive for Del E746-A750 mutation, 6 for L858R mutation. IHC analysis shows that 30 cases scored 0, 12 cases scored 2+ and 8 cases scored 3+ in Del E746-A750 mutation while 24 cases scored 0, 1 case scored 2+ and 5 cases scored 3+ in L858R mutation. The mean age of mutation positive is 56±9.50 and negative is 53.37±11.83. 65.38% male and 34.61% female were positive for EGFR mutation. The study shows that 8 (30.77%) ex/ current smokers and 18 (69.2%) nonsmokers were positive for EGFR mutations. Conclusion: Patients who were positive for EGFR mutation can take gefitinib treatment instead of taking other chemotherapy regimen. This is a rapid method for diagnosis of lung cancer patients and helpful in the treatment decision which improve the burden of the disease.

Authors and Affiliations

Priyanka Gaur| Department of Physiology King George’s Medical University, Lucknow, India, Sandeep Bhattacharya| Department of Physiology King George’s Medical University, Lucknow, India, Surya Kant| Department of Respiratory Medicine King George’s Medical University, Lucknow, India, RAS Kushwaha| Department of Respiratory Medicine King George’s Medical University, Lucknow, India, Jyotsana Agarwal| Department of Microbiology, King George’s Medical University, Lucknow, India, Anumesh Kumar Pathak | Department of Pathology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow, India

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  • EP ID EP16043
  • DOI http://dx.doi.org/10.21276/IJRDPL.2278-0238.2017.6(6).2796-2
  • Views 360
  • Downloads 18

How To Cite

Priyanka Gaur, Sandeep Bhattacharya, Surya Kant, RAS Kushwaha, Jyotsana Agarwal, Anumesh Kumar Pathak (2017). Epidermal Growth Factor Receptor Mutations detection by Mutant Specific Immunohistochemistry in North Indian Lung Cancer population. International Journal of Research and Development in Pharmacy & Life Sciences (IJRDPL), 6(6), 2796-2799. https://www.europub.co.uk/articles/-A-16043