Erythropoietic and Bone Marrow Stimulating Activity of Terminalia catappa Extract: Possible Role of Nitric Oxide Signaling
Journal Title: International Blood Research & Reviews - Year 2013, Vol 1, Issue 1
Abstract
Aims: We assessed the capacity and mechanism of Terminalia catappa (TC) to induce erythropoiesis in vivo in phenylhydrazine- induced anemic mice. Place and Duration of Study: Sample: This study was carried out at Department of Biochemistry and Center for Biotechnology Research and Training Ahmadu Bello University Zaria, and National Research Institute for Chemical Technology, Zaria. The duration spanned between Jan 2011 and Feb 2012. Methodology: Solvent fractions of Terminalia catappa aqueous extract was used to treat phynylhydrazine-induced anemic mice. Treatment was done for four days, erythropoietic activity of each fraction was assayed by determining the effect of these fractions on intracellular hemoglobin and reticulocyte level from the blood, arginase was also assayed. Bone marrow carbonic anhydrase was assayed to monitor bone marrow erythropoietic stimulation. Results: Terminalia catappa was able to up-regulate the synthesis of intracellular hemoglobin (0.135 ±0.004 μmol/0.1ml) significantly comparable to hydroxyurea (HU) (0.158±0.006 μmol/0.1ml), and normalize the peripheral blood reticulocyte index significantly at P<.05 0.94±0.25% close to the non anemic mice 0.97±0.25% and bone marrow carbonic anhydrase activity. TC inhibited arginase activity significantly (P<.05) comparable to hydroxyurea. Conclusion: The results demonstrate Terminalia catappa extract as an erythropoietic agent that supports normal erythroid differentiation in vivo in phenylhydrazine- induced anemic mice in a synergistic fashion.
Authors and Affiliations
I. A. Aimola
Keywords
Related Articles
- EP ID EP242037
- DOI 10.9734/IBRR/2013/1812
- Views 177
- Downloads 0
The Cell Based Model of in-vivo Coagulation: A Work in Progress
The goal of this article is to review the evolution of theories of coagulation and their proposed models to serve as a tool. When reviewing the research and practice literature that was published in the context of these...
Iron Status and Red Cell Parameters in Pregnant and Non-pregnant Adolescents in Côte d’Ivoire (West Africa)
Aims: The objective of this study was to evaluate iron metabolism and compare iron stores between pregnant and non-pregnant adolescents in Côte d’Ivoire. Place and Duration of Study: The study was undertaken with 187 vo...
Myoglobin Status in Stable Patients with Chronic Kidney Disease
Aim: To measure serum myoglobin concentration in stable patients with chronic kidney disease (CKD) who had not commenced hemodialysis and determine its relationship with cardiovascular risk factors. Study Design: Cross-s...
Glycated Hemoglobin Evaluation by Means of Ion Exchange Chromatography and Immunoassay in Normal and Hemoglobinopathy Patients
Aims and Objectives: High performance liquid chromatography which is basically an Ion Exchange Chromatography, is the most popular as well as expensive method for glycated hemoglobin estimation but biased results have be...
Behaviour of Fetal Haemoglobin and Its Correlation to Haemoglobin A1c Levels in Sudanese Adult Insulin-Dependent Diabetes Mellitus Patients
Aims: To estimate the fetal haemoglobin (HbF) in diabetes mellitus patients and its correlation to haemoglobin A1c levels as it is one of the effective diagnostic tools used in monitoring of diabetic patients. Study Des...