Establishment and capacity analysis of the platelet donor database with HPA and HLA genotype in Nanjing
Journal Title: Chinese Journal of Blood Transfusion - Year 2024, Vol 37, Issue 10
Abstract
Objective To establish a known HPA/HLA genotype platelet donor database in Nanjing, analyze the polymorphism of HPA-1~6w, -15 and HLA-A, -B and evaluate the matching probability and appropriate capacity of the database for platelet matching transfusion. Methods HPA-1~6w, -15 and HLA-A, -B were genotyped by PCR-SSP and PCR-SBT, respectively. The allele frequency and haplotype frequency of HPA-1~6w, -15 and HLA-A,- B were calculated with SHEsis software, and then the matching probability and appropriate capacity were obtained according to the haplotype frequencies. Results The population genetic polymorphisms data of HPA-1~6w, -15 and HLA-A, -B in Nanjing were obtained. According to the subsequent calculating, without considering ABO blood type, in a database size of 527 donors, a patient with haplotype frequency >0.001 has approximate 95% probability to achieve matching of HPA-1~6w, -15 genotype. A database with a total size of 1 875 donors can afford the patient with haplotype frequency >0.001 to find at least 1 HLA-A, -B matched donor in 95% probability. Conclusion We established a local platelet donor database with known HPA and HLA genotype, which also provided important data support for the subsequent construction, maintenance and clinical application of the database.
Authors and Affiliations
Taixiang LIU, Ling MA, Wenbiao LIANG, Nizhen JIANG, Ling LI
Keywords
Related Articles
- EP ID EP749835
- DOI 10.13303/j.cjbt.issn.1004-549x.2024.10.006
- Views 50
- Downloads 0
Blood consumption and clinical prognosis in patients with massive blood transfusion: a retrospective analysis
Objective To investigate the incidence of clinical massive blood transfusion in hospitals, the proportion of departments conducted massive blood transfusion and the current situation of component transfusion, so as to pr...
Three-dimensional genomic characterization of two multiple myeloma patients with normal karyotype and complex karyotype
[Objective] To investigate the functional differences and potential effects of chromatin spatial structure in patients with normal karyotype and complex karyotype multiple myeloma. [Methods] High-throughput chromosome co...
Hepatitis E virus prevalence among blood donors in Wuhan
Objective To investigate the prevalence and risk factor of hepatitis E virus(HEV) infection among blood donors in Wuhan. Methods A total of 1 302 serum samples (including 1 076 with normal ALT and 226 with elevated ALT)...
Study on mitochondrial respiratory function in stored apheresis platelets using real-time energy metabolism detection technology
Objective To explore the optimal parameters for detecting mitochondrial respiratory function (MRF) in apheresis platelets (AP) using real-time energy metabolism detection technology, and to study the change of MRF in AP...
Diagnostic analysis and clinical treatment strategies for patients with hyperhemolytic syndrome: a case report
Objective To analyze a case of hyperhemolytic syndrome(HHS) and explore the laboratory diagnostic method and clinical treatment strategy. Methods Serological tests such as blood typing, direct antiglobulin test(DAT), ant...