ESTABLISHMENT OF CELL SUSPENSION CULTURE FROM ONOSMA DASYTRICHUM SEED CALLUS CULTURE
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2011, Vol 2, Issue 10
Abstract
The initiation of callus production and establishment of cell suspension culture from Onosma dasytrichum are described in this study. In this study, using different plant growth media and various concentrations of sucrose and 2,4-D, the growth patterns of cell suspension cultures were examined. Murashige and Skoog medium was identified as best medium for cell suspension culture. Medium containing 2,4-D (0.2 mg/L) and 40 mg/L sucrose induced higher rates of cell division and produced more cell increase ratio for fresh weight (CIRFW) than other media which were examined.
Authors and Affiliations
SAMANE BAGERI| National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran,Faculty of Agricultural Sciences, Payame Noor University, Tehran, Iran, FOROUGH SANJARIAN*| National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran, KAMALEDIN HAGHBEEN| National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran, MOHAMMMAD ALI EBRAHIMI| Faculty of Agricultural Sciences, Payame Noor University, Tehran, Iran
Keywords
Related Articles
- EP ID EP4859
- DOI -
- Views 515
- Downloads 37
Assessing the Occupational Noise in Workplaces at Local Levels
Noise-induced hearing loss (NIHL) is a major occupational health problem that can be prevented through the use of hearing protection devices (HPDs). The objective of the paper was the accomplishment of a survey ofNoise-i...
Study of the relationship between knowledge and emotional intelligence; case study: Melli bank of Qom
In recent years, knowledge management has been known as one of the most interesting and challenging subjects in business management and its range of application is increasing along with other subjects of management. Emot...
The formation of G-super soluble Groups
A class of finite groups is called saturated formation ? when if ( ) G G ?? ? , then G ?? in which ? is called a class of groups. The purpose of this paper is to show that the finite super soluble groups form a saturated...
Evaluation of some qualitative and quantitative traits, strength and quality of safflower seeds obtained from water and dry growing conditions and different plant densities in greenhouse conditions
Strength and quality of safflower seed (Sinai cultivar) in the development and maturation of seeds, water and dry land under cultivation, were evaluated in the crop year 2010 in greenhouse of Faculty of Agriculture Unive...
Effect of Iranian Bt cotton on Encarsia formosa, parasitoid of Bemisia tabaci
Transgenic Bt cotton produces the Cry 1Ab toxin of Bacillus thuringiensis that developing against Helocoverpa armigera may be affected on secondary pest such as Bemisia tabaci. The laboratory experiments were conducted t...