Estimation of Anadara tuberculosa genetic diversity in five mangroves from Tumaco, using Cytochrome oxidase I enzyme
Journal Title: Revista MVZ Cordoba - Year 2016, Vol 21, Issue 3
Abstract
Objective. To estimate the genetic diversity of the Anadara tuberculosa en five mangrove swams of Tumaco, Nariño, Colombia using as a mitocondrial molecular marker the cytochromo oxidase sub-unit I (COI). Materials and methods. A total of 50 individuals were collected from the San Jorge, La Tiburonera, El Pajal, La Playa y Bajito Vaquería mangrove swamps, randomly selecting 10 specimens of each zone. The tissue sample was worked with absolute alcohol at ambient temperature in microtubes. DNA was extracted, and the mitocondrial DNA was amplified using the PCR technique (polymerase chain reaction). The amplified and quantified products of PCR were sequenced on both sides (Macrogen). Each one of the obtained sequences was edited and aligned. Later, the parameters of genetic diversity (haplotypical and nucleotidical) were measured, and the analysis of distribution between frequency pairs (Mistmach distribution) was elaborated. Finally, the analysis of nucleotidic variation and population structure (AMOVA) was completed. Results. The amplified product gene weighed 710 bp. The haplotypical diversity reported for all the populations was high (0.683±0.060) and the reported nucleotídical diversity was low for all the populations (0.040±0.020). The AMOVA results indicate that the variance amongst populations is low (4.20%) and that the variance within populations is high (95.80%). Conclusions. The studied populations are not structured and although there is a decrease of natural banks, the genetic diversity is high.
Authors and Affiliations
Esmeralda Chamorro L, M. Sc| Universidad de Nariño, Facultad de Ciencias Pecuarias, Departamento de Recursos Hidrobiológicos, Programa de Ingeniería en Producción Acuícola. Ciudadela Universitaria Torobajo. Pasto Colombia.Universidade Federal de Santa Catarina, Laboratório de Camarões Marinhos, Programa de pós-graduação em aquicultura, Centro de Ciências Agrarias, Brasil. CEP: 88062-601., Carol Rosero G, Ph. D *| Universidad de Nariño, Facultad de Ciencias Exactas y Naturales, Departamento de Biología. Ciudadela Universitaria Torobajo. Pasto Colombia.Correspondence: cyroga@yahoo.com
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