Estradiol Regulates Insulin Dependent Stimulation of LDL-Receptor Expression in HepG2 Cells
Journal Title: Indian Journal of Clinical Biochemistry - Year 2006, Vol 21, Issue 1
Abstract
The low density lipoprotein, one of the major vehicle for extraneous cholesterol, internalizes into the cells through the process of LDL-receptor mediated endocytosis. The expression of LDL- receptor on the cell surface is a function of various hormone regulated transcription of the receptor gene. The present study elucidates the differential expression pattern of LDL-receptor protein in human hepatoma HepG2 cells by the influence of two hormones, insulin and estrogen (β-estradiol), as compared to the basal level expression of the receptor protein . The combined effect of insulin and β-estradiol reveals that β-estradiol is the ultimate regulator between these two hormones and supershades the message of insulin on LDL-receptor expression. The receptor protein level immunobloted by anti LDL-receptor antibody after treating cells with insulin, β- estradiol and mixture of both also reflects the same phenomena. This comparative study makes it consistent that cell saturation with sterol (β-estradiol) is the prime regulator of LDLreceptor expression between the two hormones , insulin and estrogen.
Authors and Affiliations
Gopalakrishnan R. and N. C. Chandra#
Keywords
Related Articles
- EP ID EP83730
- DOI -
- Views 125
- Downloads 0
SIALIC ACID IN CARDIOVASCULAR DISEASES
Sialic acid, the acylated derivatives of 9-carbon sugar neuraminic acid, present as terminal component of oligosaccharide chains of many glycoproteins and glycolipids, has been recognized to be involved in the regulati...
SIGNIFICANCE OF TUMOR MARKERS IN LUNG CANCER
The objective was to test the utility of the cytokeratins CYFRA 21-1, tissue polypeptide specific antigen (TPS), Neuron specific enolase (NSE) and Carcino Embryonic antigen (CEA) in patients with lung cancer and in the...
PARAOXONASE ACTIVITY AND ANTIBODIES TO OXIDIZED-LDL IN CHRONIC RENAL FAILURE PATIENTS ON RENAL REPLACEMENT THERAPY
Serum paraoxonase (PON1) and antibodies to oxidized-LDL (anti ox-LDL) were measured in chronic renal failure subjects on renal replacement therapy such as hemodialysis (HD) peritoneal dialysis (PD) and transplantation...
AMBULATORY DIABETIC PATIENT WITH REMARKABLE HYPOGLYCAEMIA
DETECTION OF ENZYMES DEHYDROGENASES AND PROTEASES IN BRUGIA MALAYI FILARIAL PARASITES
Lymphatic filariasis caused mainly by infection from W. bancrofti and B. malayi remains a major cause of clinical morbidity in tropical and subtropical countries. Analysis of B.malayi mf, infective larval and adult wor...